Krzyżanowska-Gołąb Dorota, Lemańska-Perek Anna, Pupek Małgorzata, Lindner Karolina, Polańska Bożena, Porębska Irena, Kątnik-Prastowska Iwona
a Department of Chemistry and Immunochemistry , Wrocław Medical University , Wrocław , Poland.
J Immunoassay Immunochem. 2014;35(4):412-27. doi: 10.1080/15321819.2014.885448.
SDS-agarose FN immunoblotting of 257 normal and pathological human plasma samples revealed the ladder pattern of multiple plasma FN bands which corresponded to FN monomer and dimer, and 5 FN-fibrin bands with increasing molecular masses. The FN-fibrin bands of about 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa, and 1900 kDa appeared more frequently and in significantly higher relative amounts in the pathological samples (P < 0.000) than in relatively healthy individuals. The revealing of high-molecular FN-fibrin complexes by SDS-agarose FN immunobloting might have the potential to become a laboratory biomarker of some diseases in which the coagulation system is triggered.
对257份正常和病理状态的人类血浆样本进行SDS-琼脂糖纤维连接蛋白免疫印迹分析，结果显示出多条血浆纤维连接蛋白条带的梯形模式，这些条带对应于纤维连接蛋白单体和二聚体，以及5条分子量逐渐增加的纤维连接蛋白-纤维蛋白条带。约750 kDa、1000 kDa、1300 kDa、1600 kDa和1900 kDa的纤维连接蛋白-纤维蛋白条带在病理样本中出现的频率更高，相对含量也显著更高（P < 0.000），高于相对健康的个体。通过SDS-琼脂糖纤维连接蛋白免疫印迹法揭示高分子量纤维连接蛋白-纤维蛋白复合物，可能有潜力成为某些触发凝血系统疾病的实验室生物标志物。
