Department of Chemistry and Immunochemistry, Wroclaw Medical University, M. Skłodowskiej-Curie 48/50, 50-369 Wrocław, Poland.
Department of Anesthesiology and Intensive Therapy, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.
Mediators Inflamm. 2020 Aug 1;2020:8364247. doi: 10.1155/2020/8364247. eCollection 2020.
There is a pressing need for specific prognostic markers that could be used to monitor the severity of sepsis. The aims of our study were to investigate changes in the expression of different molecular forms of fibronectin in sepsis and to assess their relationship to the clinical severity and mortality of patients. . Forms of fibronectin: plasma (pFN), cellular (EDA-FN), FN-fibrin complexes, and fibronectin fragments were analyzed in 71 sepsis patients (survivors and nonsurvivors) and in the control by ELISA and immunoblotting.
The baseline pFN concentration of patients with sepsis was significantly lower than in the control (133.0 mg/L vs. 231.2 mg/L) ( < 0.001), and in nonsurvivors, it was lower than in survivors (106.0 mg/L vs. 152.8 mg/L) ( = 0.004). The baseline EDA-FN was significantly elevated in both sepsis groups (survivors: 6.7 mg/L; nonsurvivors: 9.4 mg/L) compared to the control (1.4 mg/L) ( < 0.001). It should be noted that among patients with more severe sepsis, the EDA-FN level was higher in nonsurvivors than in survivors. Furthermore, molecular FN-fibrin complexes as well as FN fragments occurred much more frequently in nonsurvivors than in survivors.
The study showed that in sepsis, changes in plasmatic and cellular form of fibronectin were associated with the severity of sepsis and may be useful predictors of outcome.
目前迫切需要能够用于监测脓毒症严重程度的特定预后标志物。我们的研究旨在探讨不同形式纤维连接蛋白在脓毒症中的表达变化，并评估其与患者临床严重程度和死亡率的关系。通过 ELISA 和免疫印迹法分析了 71 例脓毒症患者（存活者和非存活者）和对照组中纤维连接蛋白的不同分子形式（血浆 [pFN]、细胞 [EDA-FN]、FN-纤维蛋白复合物和纤维连接蛋白片段）。
脓毒症患者的基线 pFN 浓度明显低于对照组（133.0mg/L 比 231.2mg/L）（ < 0.001），且非存活者低于存活者（106.0mg/L 比 152.8mg/L）（ = 0.004）。两组脓毒症患者的基线 EDA-FN 均明显升高（存活者：6.7mg/L；非存活者：9.4mg/L），高于对照组（1.4mg/L）（ < 0.001）。值得注意的是，在脓毒症严重程度较高的患者中，非存活者的 EDA-FN 水平高于存活者。此外，分子 FN-纤维蛋白复合物以及 FN 片段在非存活者中比在存活者中更频繁地发生。
本研究表明，在脓毒症中，纤维连接蛋白的血浆和细胞形式的变化与脓毒症的严重程度相关，可能是预后的有用预测指标。
