1] State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Nat Commun. 2014;5:3352. doi: 10.1038/ncomms4352.
Loss of seed dispersal is a key agronomical trait targeted by ancient human selection and has been regarded as a milestone of crop domestication. In this study, in the legume crop soybean Glycine max (L.) Merr. which provides vegetable oils and proteins for humans, we show that the key cellular feature of the shattering-resistant trait lies in the excessively lignified fibre cap cells (FCC) with the abscission layer unchanged in the pod ventral suture. We demonstrate that a NAC (NAM, ATAF1/2 and CUC2) gene shattering1-5 (SHAT1-5) functionally activates secondary wall biosynthesis and promotes the significant thickening of FCC secondary walls by expression at 15-fold the level of the wild allele, which is attributed to functional disruption of the upstream repressor. We show that strong artificial selection of SHAT1-5 has caused a severe selective sweep across ~ 116 kb on chromosome 16. This locus and regulation mechanism could be applicable to legume crop improvement.
丧失种子传播能力是古代人类选择的一个关键农艺性状，被认为是作物驯化的一个里程碑。在本研究中，在为人类提供植物油和蛋白质的豆科作物大豆 Glycine max（L.）Merr. 中，我们发现抗裂荚特性的关键细胞特征在于纤维帽细胞（FCC）过度木质化，荚果腹缝线中的离层没有变化。我们证明一个 NAC（NAM、ATAF1/2 和 CUC2）基因 shattering1-5（SHAT1-5）通过在野生等位基因水平上表达 15 倍的水平，功能性地激活了次生壁生物合成，并促进了 FCC 次生壁的显著增厚，这归因于上游抑制剂的功能破坏。我们表明，SHAT1-5 的强烈人工选择导致了在染色体 16 上约 116kb 的严重选择清除。该基因座和调控机制可应用于豆科作物的改良。
