Department of Electronics, Carleton University, Ottawa, ON, Canada; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Biosens Bioelectron. 2014 Jun 15;56:359-67. doi: 10.1016/j.bios.2014.01.018. Epub 2014 Jan 18.
This paper reports on the application of an optical fiber biosensor for real-time analysis of cellular behavior. Our findings illustrate that a fiber sensor fabricated from a traditional telecommunication fiber can be integrated into conventional cell culture equipment and used for real-time and label-free monitoring of cellular responses to chemical stimuli. The sensing mechanism used for the measurement of cellular responses is based on the excitation of surface plasmon resonance (SPR) on the surface of the optical fiber. In this proof of concept study, the sensor was utilized to investigate the influence of a number of different stimuli on cells-we tested the effects of trypsin, serum and sodium azide. These stimuli induced detachment of cells from the sensor surface, uptake of serum and inhibition of cellular metabolism, accordingly. The effects of different stimuli were confirmed with alamar blue assay, phase contrast and fluorescence microscopy. The results indicated that the fiber biosensor can be successfully utilized for real-time and label-free monitoring of cellular response in the first 30 min following the introduction of a stimulus. Furthermore, we demonstrated that the optical fiber biosensors can be easily regenerated for repeated use, proving this platform as a versatile and cost-effective sensing tool.
本文报告了一种光纤生物传感器在实时分析细胞行为中的应用。我们的研究结果表明，由传统通信光纤制成的光纤传感器可以集成到常规细胞培养设备中，用于实时、无标记监测细胞对化学刺激的反应。用于测量细胞反应的传感机制基于光纤表面的表面等离子体共振（SPR）的激发。在这个概念验证研究中，该传感器用于研究多种不同刺激对细胞的影响-我们测试了胰蛋白酶、血清和叠氮化钠的影响。这些刺激分别导致细胞从传感器表面脱落、摄取血清和抑制细胞代谢。通过台盼蓝检测、相差和荧光显微镜证实了不同刺激的效果。结果表明，光纤生物传感器可成功用于在引入刺激后的头 30 分钟内实时、无标记监测细胞反应。此外，我们证明光纤生物传感器可以轻松再生，可重复使用，证明该平台是一种多功能且具有成本效益的传感工具。
