理解纤维素可及性在木质纤维素底物酶水解中的作用的最新进展。
Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates.
机构信息
BioEnergy Science Center, School of Chemistry and Biochemistry, Institute of Paper Science and Technology, Georgia Institute of Technology, 500 10th Street, Atlanta, GA 30332, USA.
BioEnergy Science Center, School of Chemistry and Biochemistry, Institute of Paper Science and Technology, Georgia Institute of Technology, 500 10th Street, Atlanta, GA 30332, USA.
出版信息
Curr Opin Biotechnol. 2014 Jun;27:150-8. doi: 10.1016/j.copbio.2014.01.014. Epub 2014 Feb 16.
Cellulose accessibility has been proposed as a key factor in the efficient bio-conversion of lignocellulosic biomass to fermentable sugars. Factors affecting cellulose accessibility can be divided into direct factors that refer to accessible surface area of cellulose, and indirect factors referring to chemical composition such as lignin/hemicellulose content, and biomass structure-relevant factors (i.e. particle size, porosity). An overview of the current pretreatment technologies special focus on the major mode of action to increase cellulose accessibility as well as multiple techniques that could be used to assess the cellulose accessibility are presented in this review. The appropriate determination of cellulose accessibility before and after pretreatment can assist to understand the effectiveness of a particular pretreatment in overcoming lignocellulosic recalcitrance to improve substrate enzymatic digestibility.
纤维素可及性被认为是木质纤维素生物质高效生物转化为可发酵糖的关键因素。影响纤维素可及性的因素可分为直接因素，即纤维素的可及表面积，以及间接因素，如木质素/半纤维素含量，和与生物质结构相关的因素（即粒径、孔隙率）。本文综述了目前的预处理技术，特别关注提高纤维素可及性的主要作用模式，以及可用于评估纤维素可及性的多种技术。在预处理前后适当地确定纤维素可及性有助于理解特定预处理克服木质纤维素抗降解性以提高底物酶解性的有效性。
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