Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, China.
Int J Mol Sci. 2023 Aug 30;24(17):13422. doi: 10.3390/ijms241713422.
Xylo-oligosaccharides (XOS) enriched with high fractions of X2-X3 are regarded as an effective prebiotic for regulating the intestinal microflora. In this study, the original XOS solution was obtained from bamboo shoots through hydrothermal pretreatment under optimized conditions. Subsequently, enzymatic hydrolysis with endo-xylanase was performed on the original XOS solution to enhance the abundance of the X2-X3 fractions. The results demonstrated that hydrothermal pretreatment yielded 21.24% of XOS in the hydrolysate solution, and subsequent enzymatic hydrolysis significantly increased the proportion of the X2-X3 fractions from 38.87% to 68.21%. Moreover, the XOS solutions with higher amounts of X2-X3 fractions exhibited superior performance in promoting the growth of probiotics such as and in vitro, leading to increased production of short-chain fatty acids. In the in vivo colitis mouse model, XOS solutions with higher contents of X2-X3 fractions demonstrated enhanced efficacy against intestinal inflammation. Compared with the colitis mice (model group), the XOS solution with higher X2-X3 fractions (S1 group) could significantly increase the number of in the intestinal microflora, while the original XOS solution (S2 group) could significantly increase the number of in the intestinal microflora of colitis mice. In addition, the abundances of and in the intestinal microflora of the S1 and S2 groups were much lower than in the model group. This effect was attributed to the ability of these XOS solutions to enhance species diversity, reversing the imbalance and disorder within the intestinal microflora. Overall, this work highlights the outstanding potential of XOS enriched with high contents of X2-X3 fractions as a regulator of the intestinal microbiota and as an anti-colitis agent.
木二糖（XOS）富含高比例的 X2-X3，被认为是一种有效的调节肠道菌群的益生元。在本研究中，原始 XOS 溶液是通过优化条件下的竹材热水预处理从竹笋中获得的。然后，用内切木聚糖酶对原始 XOS 溶液进行酶解，以提高 X2-X3 部分的丰度。结果表明，热水预处理在水解液中得到 21.24%的 XOS，随后的酶解显著提高了 X2-X3 部分的比例从 38.87%到 68.21%。此外，具有更高比例 X2-X3 部分的 XOS 溶液在体外更能促进益生菌如 和 的生长，导致短链脂肪酸的产生增加。在体内结肠炎小鼠模型中，具有更高比例 X2-X3 部分的 XOS 溶液对肠道炎症的疗效增强。与结肠炎小鼠（模型组）相比，具有更高 X2-X3 部分的 XOS 溶液（S1 组）可显著增加肠道微生物群中的 数量，而原始 XOS 溶液（S2 组）可显著增加结肠炎小鼠肠道微生物群中的 数量。此外，S1 和 S2 组肠道微生物群中 和 的丰度远低于模型组。这种效果归因于这些 XOS 溶液增强物种多样性的能力，逆转了肠道微生物群的失衡和紊乱。总的来说，这项工作突出了富含高比例 X2-X3 部分的 XOS 作为肠道微生物群调节剂和抗结肠炎剂的巨大潜力。
