Logan K A, Dahmus M E, Bradbury E M
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
J Biol Chem. 1988 Jul 15;263(20):9658-62.
We report here the cross-reaction of RNA polymerase II antiserum with histones H1(0) and H5 and the complementary cross-reactions of antisera to the globular domain of histone H1(0) (GH1(0)) and histone H5 (GH5) with RNA polymerase II. Immunoblotting of RNA polymerase II antiserum with fragments of histone H1(0) localized the cross-reaction at the junction of the globular and C-terminal domains of histone H1(0). The structural homology implied by these cross-reactions is interesting in light of reports that suggest H1(0) may play a role in differentiation and development.
我们在此报告RNA聚合酶II抗血清与组蛋白H1(0)和H5的交叉反应，以及针对组蛋白H1(0)球状结构域（GH1(0)）和组蛋白H5（GH5）的抗血清与RNA聚合酶II的互补交叉反应。用组蛋白H1(0)片段对RNA聚合酶II抗血清进行免疫印迹分析，将交叉反应定位在组蛋白H1(0)球状结构域与C末端结构域的交界处。鉴于有报道表明H1(0)可能在分化和发育中起作用，这些交叉反应所暗示的结构同源性很有意思。
