Manesh Sara Shakeri, Deperas-Kaminska Marta, Fotouhi Asal, Sangsuwan Traimate, Harms-Ringdahl Mats, Wojcik Andrzej, Haghdoost Siamak
Department of Molecular Biosciences, Centre for Radiation Protection Research (CRPR), The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden.
Radiat Environ Biophys. 2014 May;53(2):417-25. doi: 10.1007/s00411-014-0521-1. Epub 2014 Feb 19.
The aim of the present study was to analyse the dose rate effect of gamma radiation at the level of mutations, chromosomal aberrations, and cell growth in TK6 cells with normal as well as reduced levels of hMTH1 protein. TK6 cells were exposed to gamma radiation at dose rates ranging from 1.4 to 30.0 mGy/h (chronic exposure) as well as 24 Gy/h (acute exposure). Cell growth, frequency of thymidine kinase mutants, and of chromosomal aberrations in painted chromosomes 2, 8, and 14 were analysed. A decline in cell growth and an increase in unstable-type chromosomal aberrations with increasing dose rate were observed in both cell lines. A dose rate effect was not seen on mutations or stable-type chromosomal aberrations in any of the two cell lines. Reduction in the hMTH1 protein does not influence the sensitivity of TK6 cells to gamma radiation. This result fits well with data of others generated with the same cell line.
本研究的目的是分析在具有正常水平以及降低水平hMTH1蛋白的TK6细胞中，γ辐射在突变、染色体畸变和细胞生长水平上的剂量率效应。将TK6细胞暴露于剂量率范围为1.4至30.0 mGy/h（慢性暴露）以及24 Gy/h（急性暴露）的γ辐射下。分析细胞生长、胸苷激酶突变体频率以及2号、8号和14号染色体涂染染色体中的染色体畸变情况。在两种细胞系中均观察到随着剂量率增加，细胞生长下降以及不稳定型染色体畸变增加。在两种细胞系中的任何一种中，均未观察到剂量率对突变或稳定型染色体畸变有影响。hMTH1蛋白的减少并不影响TK6细胞对γ辐射的敏感性。该结果与使用相同细胞系产生的其他数据非常吻合。
