Oldach K, Morgenstern A, Rother S, Girgi M, O'Kennedy M, Lörz H
Institute for Applied Molecular Plant Biology, AMPII, University of Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany, Germany.
Plant Cell Rep. 2001 Jul;20(5):416-421. doi: 10.1007/s002990100335. Epub 2014 Feb 15.
We report here an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated 10-20 days after pollination and cultured on various L3 media. The influence of different parameters during the callus induction phase was examined with respect to the regeneration rate: (1) the concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and various cytokinins; (2) the addition of AgNO; (3) the use of maltose or sucrose as a carbon source. Modifications in the phytohormones alone resulted in the regeneration of fertile sorghum plants at high efficiency. Significant increases in the regeneration rates of pearl millet genotypes were achieved by the combination of sucrose as a carbon source and silver nitrate as a potential ethylene inhibitor.
我们在此报告一种体外培养系统，该系统能从珍珠粟[Pennisetum glaucum (L.) R. Br.]和高粱[Sorghum bicolor (L.) Moench]的未成熟胚中实现可靠且高效的再生。在授粉后10 - 20天分离未成熟胚，并在各种L3培养基上进行培养。在愈伤组织诱导阶段，针对再生率研究了不同参数的影响：(1) 2,4 - 二氯苯氧乙酸(2,4 - D)和各种细胞分裂素的浓度；(2) 添加硝酸银；(3) 使用麦芽糖或蔗糖作为碳源。仅植物激素的改变就能高效再生可育的高粱植株。通过将蔗糖作为碳源与硝酸银作为潜在的乙烯抑制剂相结合，显著提高了珍珠粟基因型的再生率。
