Scibior Agnieszka, Adamczyk Agnieszka, Gołębiowska Dorota, Niedźwiecka Irmina, Fornal Emilia
Laboratory of Oxidative Stress, Center for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Kraśnicka Ave. 102, 20-718 Lublin, Poland.
Metallomics. 2014 Apr;6(4):907-20. doi: 10.1039/c3mt00363a.
The effect of 12 week co-administration of sodium metavanadate (SMV) and magnesium sulfate (MS) on the levels of some elements in selected rats' organs and an attempt to elucidate a role of divalent metal transporter 1 (DMT-1) in the mechanism(s) of the SMV-induced disorders in some tissue Fe homeostasis were studied. SMV taken up separately or in combination with MS may pose a risk of the rise and shortage of the total hepatic and splenic Fe and Cu contents, respectively, cerebral Fe deficiency, splenic Ca deposition, and the hepatic, renal, and cerebral DMT-1 down-regulation. When administered alone, SMV may also cause the decrease in the total renal Fe and Cu contents. A visible protective effect of Mg against the renal and cerebral V accumulation and the decrease in the renal Fe and Cu contents during the SMV-MS co-administration together with our previous findings suggest a beneficial role of Mg at SMV exposure. Further, the SMV-induced fall in total iron binding capacity (TIBC), reported previously, and its correlations with the hepatic, splenic, and cerebral Fe levels allow us to suggest that diminished TIBC could be partly involved in the mechanism(s) responsible for the dramatic redistribution of Fe in those tissues. Finally, DMT-1, which potentially could participate in the hepatic non-transferrin Fe-bound uptake, does not play a significant role in this process indicating the need for studying other Fe transporters to more precisely elucidate molecular mechanism(s) underlying the hepatic Fe loading in our experimental conditions.
研究了偏钒酸钠（SMV）与硫酸镁（MS）联合给药12周对所选大鼠器官中某些元素水平的影响，并试图阐明二价金属转运蛋白1（DMT-1）在SMV诱导的某些组织铁稳态紊乱机制中的作用。单独或与MS联合使用的SMV可能分别导致肝脏和脾脏中铁和铜总量升高和缺乏的风险，脑铁缺乏，脾脏钙沉积以及肝脏、肾脏和大脑中DMT-1的下调。单独给药时，SMV也可能导致肾脏中铁和铜的总量减少。镁对SMV-MS联合给药期间肾脏和大脑中钒的积累以及肾脏中铁和铜含量的降低具有明显的保护作用，这与我们之前的研究结果表明镁在SMV暴露时具有有益作用。此外，先前报道的SMV引起的总铁结合能力（TIBC）下降及其与肝脏、脾脏和大脑中铁水平的相关性使我们认为，TIBC降低可能部分参与了这些组织中铁显著重新分布的机制。最后，可能参与肝脏中非转铁蛋白结合铁摄取的DMT-1在这一过程中不发挥重要作用，这表明需要研究其他铁转运蛋白，以更精确地阐明我们实验条件下肝脏铁负荷的分子机制。
