前体信使核糖核酸剪接反应。

The pre-mRNA splicing reaction.

作者信息

Wongpalee Somsakul Pop, Sharma Shalini

机构信息

Department of Immunology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA, USA.

出版信息

Methods Mol Biol. 2014;1126:3-12. doi: 10.1007/978-1-62703-980-2_1.

DOI:10.1007/978-1-62703-980-2_1

PMID:24549652

Abstract

In eukaryotic organisms, nascent transcripts of protein-coding genes contain intronic sequences that are not present in mature mRNAs. Pre-mRNA splicing removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This process occurs with a high degree of accuracy to generate the functional transcriptome of a cell.

摘要

在真核生物中，蛋白质编码基因的新生转录本包含成熟mRNA中不存在的内含子序列。前体mRNA剪接去除内含子并连接外显子以形成成熟mRNA。它由一种称为剪接体的大型核糖核蛋白复合体催化。前体mRNA中的序列决定内含子的识别和切除。这个过程以高度的准确性发生，以产生细胞的功能转录组。