Wongpalee Somsakul Pop, Sharma Shalini
Department of Immunology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles, CA, USA.
Methods Mol Biol. 2014;1126:3-12. doi: 10.1007/978-1-62703-980-2_1.
In eukaryotic organisms, nascent transcripts of protein-coding genes contain intronic sequences that are not present in mature mRNAs. Pre-mRNA splicing removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This process occurs with a high degree of accuracy to generate the functional transcriptome of a cell.
在真核生物中，蛋白质编码基因的新生转录本包含成熟mRNA中不存在的内含子序列。前体mRNA剪接去除内含子并连接外显子以形成成熟mRNA。它由一种称为剪接体的大型核糖核蛋白复合体催化。前体mRNA中的序列决定内含子的识别和切除。这个过程以高度的准确性发生，以产生细胞的功能转录组。
