Morrison L A, Braciale V L, Braciale T J
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
J Immunol. 1988 Jul 15;141(2):363-8.
The induction of class I and class II MHC-restricted CTL in response to different forms of A/JAP/57 influenza virus was compared. Splenocytes removed from influenza-immune BALB/c mice and stimulated in vitro with infected syngeneic splenocytes are mainly CD8+ (Lyt-2+) and specifically lyse infected Ia- and Ia+ target cells. To a lesser extent they also lyse non-infectious virus-pulsed Ia+ but not Ia- target cells. In contrast, syngeneic stimulators pulsed with non-infectious virus (exogenous Ag) induce effector T cells that specifically lyse both infected and non-infectious virus-pulsed Ia+ target cells. The cells present in this heterogeneous culture predominantly express the CD4 (L3T4) cell surface marker. Frequency analysis by limiting dilution of splenocytes derived directly from influenza-immune mice revealed a similar pattern of precursor induction: In vitro stimulation with infected splenocytes yielded primarily class I MHC-restricted CTL, whereas stimulation with non-infectious virus reciprocally induced primarily class II MHC-restricted CTL. Thus, the Ag form and consequently the intracellular route of viral Ag presentation profoundly influence the MHC restriction of CTL precursors induced.
比较了针对不同形式的A/JAP/57流感病毒诱导的I类和II类MHC限制性CTL。从流感免疫的BALB/c小鼠中取出的脾细胞，在体外与感染的同基因脾细胞一起刺激，主要是CD8 +（Lyt-2 +），并特异性裂解感染的Ia +和Ia-靶细胞。在较小程度上，它们也裂解非感染性病毒脉冲的Ia +但不裂解Ia-靶细胞。相反，用非感染性病毒（外源性Ag）脉冲的同基因刺激物诱导效应T细胞，其特异性裂解感染的和非感染性病毒脉冲的Ia +靶细胞。这种异质培养物中存在的细胞主要表达CD4（L3T4）细胞表面标志物。通过对直接来自流感免疫小鼠的脾细胞进行有限稀释的频率分析揭示了类似的前体诱导模式：用感染的脾细胞进行体外刺激主要产生I类MHC限制性CTL，而用非感染性病毒刺激则主要诱导II类MHC限制性CTL。因此，Ag形式以及病毒Ag呈递的细胞内途径深刻影响诱导的CTL前体的MHC限制。
