Demkowicz W E, Ennis F A
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
J Virol. 1993 Mar;67(3):1538-44. doi: 10.1128/JVI.67.3.1538-1544.1993.
Stimulation of human vaccinia virus immune peripheral blood mononuclear cells in vitro from vaccinia virus-immune donors with live vaccinia virus-infected autologous cells generated vaccinia virus-specific cytotoxic T lymphocytes (CTL) capable of lysing vaccinia virus-infected cells. We generated vaccinia virus-specific CD8+ clones and CD4+ CTL lines by limiting dilution from two donors by using peripheral blood mononuclear cells obtained 2 months or 4 years postrevaccination with vaccinia virus. These results demonstrate that vaccinia virus-specific CTL are generated as a result of immunization of humans with vaccinia virus and that both CD8(+)- and CD4(+)-specific T cells are maintained as memory cells.
用活的痘苗病毒感染的自体细胞体外刺激来自痘苗病毒免疫供体的人痘苗病毒免疫外周血单核细胞，可产生能够裂解痘苗病毒感染细胞的痘苗病毒特异性细胞毒性T淋巴细胞（CTL）。我们通过有限稀释法，从两名供体中分别使用接种痘苗病毒后2个月或4年获得的外周血单核细胞，产生了痘苗病毒特异性CD8 +克隆和CD4 + CTL系。这些结果表明，痘苗病毒特异性CTL是人类接种痘苗病毒免疫的结果，并且CD8（+）和CD4（+）特异性T细胞均作为记忆细胞得以维持。
