Wu Shijin, Zhang Huaxing, Yu Xiang, Qiu Lequan
College of Biological and Environmental Engineering, Zhejiang University of Technology , Hangzhou, People's Republic of China .
Ningbo Institute of Technology, Zhejiang University , Ningbo, People's Republic of China .
Environ Eng Sci. 2014 Jan 1;31(1):9-17. doi: 10.1089/ees.2013.0038.
The aim of this study was to evaluate the acute toxicity effects of dichloromethane and dichloroethane on at the physiological and molecular level. Data showed that the cell number, chlorophyll a, and total protein content gradually decreased with increasing dichloromethane and dichloroethane concentrations over a 96-h exposure. Lower doses of two organic solvents had stimulatory effects on catalase and superoxide dismutase activity. Malondialdehyde showed a concentration-dependent increase in response to dichloromethane and dichloroethane exposure. Electron microscopy also showed that there were some chloroplast abnormalities in response to different concentrations of dichloromethane and dichloroethane exposure. Real-time polymerase chain reaction assay demonstrated that dichloromethane and dichloroethane reduced the transcript abundance of B, whereas that of C changed depending on the toxicant after 24 h of exposure. Dichloromethane and dichloroethane affected the activity of antioxidant enzymes, disrupted the chloroplast ultrastructure, and reduced transcription of photosynthesis-related genes in , leading to metabolic disruption and cell death.
本研究的目的是在生理和分子水平上评估二氯甲烷和二氯乙烷的急性毒性作用。数据显示，在96小时的暴露期间，随着二氯甲烷和二氯乙烷浓度的增加，细胞数量、叶绿素a和总蛋白含量逐渐下降。较低剂量的两种有机溶剂对过氧化氢酶和超氧化物歧化酶活性有刺激作用。丙二醛显示出对二氯甲烷和二氯乙烷暴露的浓度依赖性增加。电子显微镜还显示，对不同浓度的二氯甲烷和二氯乙烷暴露有一些叶绿体异常。实时聚合酶链反应分析表明，二氯甲烷和二氯乙烷降低了B的转录丰度，而C的转录丰度在暴露24小时后根据毒物的不同而变化。二氯甲烷和二氯乙烷影响抗氧化酶的活性，破坏叶绿体超微结构，并降低光合作用相关基因的转录，导致代谢紊乱和细胞死亡。
