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RNA amplification with transcript sequencing (RAWTS).

作者信息

Sarkar G, Sommer S S

机构信息

Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.

出版信息

Nucleic Acids Res. 1988 Jun 10;16(11):5197. doi: 10.1093/nar/16.11.5197.

DOI:10.1093/nar/16.11.5197
PMID:2455280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336733/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e9/336733/3a8cc79a8ebb/nar00154-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e9/336733/3a8cc79a8ebb/nar00154-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e9/336733/3a8cc79a8ebb/nar00154-0444-a.jpg

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Specific amplification with PCR of a refractory segment of genomic DNA.利用聚合酶链式反应（PCR）对基因组DNA的难扩增片段进行特异性扩增。

本文引用的文献

1
Genomic amplification with transcript sequencing.基因组扩增与转录本测序
Science. 1988 Jan 29;239(4839):491-4. doi: 10.1126/science.3340835.
Nucleic Acids Res. 1988 Dec 23;16(24):11844. doi: 10.1093/nar/16.24.11844.
4
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.基于转录的扩增系统及采用基于微珠的夹心杂交法检测1型人类免疫缺陷病毒的扩增产物
Proc Natl Acad Sci U S A. 1989 Feb;86(4):1173-7. doi: 10.1073/pnas.86.4.1173.
5
Role of the zeta chain in the expression of the T cell antigen receptor: genetic reconstitution studies.ζ链在T细胞抗原受体表达中的作用：基因重组研究
EMBO J. 1989 Dec 1;8(12):3651-6. doi: 10.1002/j.1460-2075.1989.tb08539.x.
6
The polymerase chain reaction: an improved method for the analysis of nucleic acids.聚合酶链反应：一种用于核酸分析的改进方法。
Hum Genet. 1989 Aug;83(1):1-15. doi: 10.1007/BF00274139.
7
Characterization of single base substitutions in edited apolipoprotein B transcripts.编辑后的载脂蛋白B转录本中单碱基替换的特征分析。
Nucleic Acids Res. 1989 Jan 25;17(2):691-8. doi: 10.1093/nar/17.2.691.
8
Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.通过表达盒聚合酶链反应对蛋白质进行过量表达和剖析。
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1937-41. doi: 10.1073/pnas.87.5.1937.
9
A new point mutation in the beta-hexosaminidase alpha subunit gene responsible for infantile Tay-Sachs disease in a non-Jewish Caucasian patient (a Kpn mutant).
Am J Hum Genet. 1990 Sep;47(3):568-74.
10
GM2-gangliosidosis B1 variant: analysis of beta-hexosaminidase alpha gene abnormalities in seven patients.GM2神经节苷脂贮积症B1变异型：7例患者β-己糖胺酶α基因异常分析
Am J Hum Genet. 1990 Feb;46(2):329-39.