Chavasco Juliana Moscardini, Prado E Feliphe Bárbara Helena Muniz, Cerdeira Claudio Daniel, Leandro Fabrício Damasceno, Coelho Luiz Felipe Leomil, Silva Jéferson Junior da, Chavasco Jorge Kleber, Dias Amanda Latercia Tranches
Biomedical Science Institute, Microbiology and Immunology Department, Federal University of Alfenas, AlfenasMG, Brazil, Biomedical Science Institute, Microbiology and Immunology Department, Federal University of Alfenas, Alfenas, MG, Brazil.
Rev Inst Med Trop Sao Paulo. 2014 Jan-Feb;56(1):13-20. doi: 10.1590/S0036-46652014000100002.
The antimicrobial activity of plant hidroethanolic extracts on bacteria Gram positive, Gram negative, yeasts, Mycobacterium tuberculosis H37 and Mycobacterium bovis was evaluated by using the technique of Agar diffusion and microdilution in broth. Among the extracts evaluated by Agar diffusion, the extract of Bidens pilosa leaf presented the most expressive average of haloes of growth inhibition to the microorganisms, followed by the extract of B. pilosa flower, of Eugenia pyriformis' leaf and seed, of Plinia cauliflora leaf which statistically presented the same average of haloes inhibitory formation on bacteria Gram positive, Gram negative and yeasts. The extracts of Heliconia rostrata did not present activity. Mycobacterium tuberculosis H37 and Mycobacterium bovis (BCG) appeared resistant to all the extracts. The susceptibility profile of Candida albicans and Saccharomyces cerevisiae fungi were compared to one another and to the Gram positive Bacillus subtilis, Enterococcus faecalis and the Gram negative Salmonella typhimurium bacteria (p > 0.05). The evaluation of cytotoxicity was carried out on C6-36 larvae cells of the Aedes albopictus mosquito. The extracts of stem and flower of Heliconia rostrata, leaf and stem of Plinia cauliflora, seed of Anonna crassiflora and stem, flower and root of B. pilosa did not present toxicity in the analyzed concentrations. The highest rates of selectivity appeared in the extracts of stem of A. crassiflora and flower of B. pilosa to Staphylococcus aureus, presenting potential for future studies about a new drug development.
采用琼脂扩散法和肉汤微量稀释法，评估了植物水乙醇提取物对革兰氏阳性菌、革兰氏阴性菌、酵母菌、结核分枝杆菌H37和牛分枝杆菌的抗菌活性。在通过琼脂扩散法评估的提取物中，三叶鬼针草叶提取物对微生物的生长抑制晕圈平均表现最为显著，其次是三叶鬼针草花提取物、洋蒲桃叶和种子提取物、巴西番樱桃叶提取物，它们在革兰氏阳性菌、革兰氏阴性菌和酵母菌上形成的抑制晕圈平均在统计学上相同。蝎尾蕉提取物没有活性。结核分枝杆菌H37和牛分枝杆菌（卡介苗）对所有提取物均表现出抗性。白色念珠菌和酿酒酵母菌的药敏谱相互比较，并与革兰氏阳性菌枯草芽孢杆菌、粪肠球菌以及革兰氏阴性菌鼠伤寒沙门氏菌进行比较（p>0.05）。对白纹伊蚊的C6-36幼虫细胞进行了细胞毒性评估。蝎尾蕉茎和花提取物、巴西番樱桃叶和茎提取物、番荔枝种子提取物以及三叶鬼针草茎、花和根提取物在所分析浓度下均未表现出毒性。番荔枝茎提取物和三叶鬼针草花提取物对金黄色葡萄球菌的选择性率最高，为未来新药开发研究提供了潜力。
