Pietrosiuk A, Sykłowska-Baranek K, Wiedenfeld H, Wolinowska R, Furmanowa M, Jaroszyk E
Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Plant Cell Rep. 2006 Oct;25(10):1052-8. doi: 10.1007/s00299-006-0161-2. Epub 2006 May 3.
Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g(-1) DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g(-1) DW) and IBS (0.307 mg g(-1) DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots.
使用三株发根农杆菌（ATCC 15834、LBA 9402和NCIB 8196）建立了毛叶紫筒草的毛状根培养体系。由发根农杆菌ATCC 15834感染产生的8个株系表现出足够的生物量增加，并进行了进一步研究。转化毛状根中乙酰紫草素（ACS）和异丁酰紫草素（IBS）的含量约为毛叶紫筒草天然根中观察到含量的10%（分别为24.35和14.48 mg g(-1)干重）。一个株系Lc1-D产生了最大量的ACS（2.72 mg g(-1)干重）和IBS（0.307 mg g(-1)干重）。在所有转化毛状根株系中均发现了痕量的吡咯里西啶生物碱（PA）、毛叶紫筒草碱和毛叶紫筒草宁碱。
