一种针对犬瘟热病毒截短型N蛋白（氨基酸277 - 471）的单克隆抗体。

A monoclonal antibody against truncated N protein (aa 277-471) of canine distemper virus.

作者信息

Yi Li, Cheng Shipeng

机构信息

Institute of Special Wild Economic Animal and Plant Science , Chinese Academy of Agricultural Sciences, Changchun, Jilin Province, P.R. China .

出版信息

Monoclon Antib Immunodiagn Immunother. 2014 Feb;33(1):52-6. doi: 10.1089/mab.2013.0066.

DOI:10.1089/mab.2013.0066

PMID:24555938

Abstract

Canine distemper (CD) is a highly contagious, systemic, viral disease of dogs seen worldwide. The nucleocapsid protein (NP) of canine distemper virus encloses virus assembly and has some regulatory functions in viral transcription and replication. Here, we describe a procedure to generate a monoclonal antibody (MAb) against CDV N protein and investigate its characteristics. Western blot analysis showed that the MAbs produced in this study were against CDV N specifically. Indirect immunofluorescence assay demonstrated that they could recognize native N protein in CDV-infected Vero cells. The MAbs reported here may provide valuable tools for the further exploration of biological properties and functions of N protein and may also be developed for potential clinical applications.

摘要

犬瘟热（CD）是一种在全球范围内可见的犬类高度传染性全身性病毒性疾病。犬瘟热病毒的核衣壳蛋白（NP）参与病毒组装，并在病毒转录和复制中具有一些调节功能。在此，我们描述了一种制备抗犬瘟热病毒N蛋白单克隆抗体（MAb）并研究其特性的方法。蛋白质免疫印迹分析表明，本研究中产生的单克隆抗体特异性针对犬瘟热病毒N蛋白。间接免疫荧光试验证明，它们能够识别犬瘟热病毒感染的Vero细胞中的天然N蛋白。本文报道的单克隆抗体可能为进一步探索N蛋白的生物学特性和功能提供有价值的工具，也可能开发用于潜在的临床应用。