针对犬瘟热病毒核衣壳蛋白的单克隆抗体的特性分析

Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein.

作者信息

Masuda Munemitsu, Sato Hiroki, Kamata Hiroshi, Katsuo Tomoe, Takenaka Akiko, Miura Ryuichi, Yoneda Misako, Tsukiyama-Kohara Kyoko, Mizumoto Kiyohisa, Kai Chieko

机构信息

Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641, Japan.

出版信息

Comp Immunol Microbiol Infect Dis. 2006 Mar-May;29(2-3):157-65. doi: 10.1016/j.cimid.2006.03.004.

DOI:10.1016/j.cimid.2006.03.004

PMID:16697903

Abstract

We have established four monoclonal antibodies (MAbs) against the nucleocapsid protein (NP) of canine distemper virus (CDV). A competitive binding assay has revealed that the MAbs are directed against two antigenic domains. An immunofluorescence assay using a series of deletion clones of the NP and an immunoprecipitation assay using the NP have revealed that two of the MAbs recognize the C-terminal region of the NP while the other two recognize the tertiary structure of the N-terminal domain. These MAbs reacted with all eight strains of CDV used in this study, but showed different reactivities against measles virus and rinderpest virus.

摘要

我们已经制备了四种针对犬瘟热病毒（CDV）核衣壳蛋白（NP）的单克隆抗体（MAb）。竞争性结合试验表明，这些单克隆抗体针对两个抗原结构域。使用NP的一系列缺失克隆进行的免疫荧光试验以及使用NP进行的免疫沉淀试验表明，其中两种单克隆抗体识别NP的C末端区域，而另外两种识别N末端结构域的三级结构。这些单克隆抗体与本研究中使用的所有八种CDV毒株发生反应，但对麻疹病毒和牛瘟病毒表现出不同的反应性。