Camargo Isabel Cristina Cherici, Leite Gabriel Adan Araújo, Pinto Tiago, Ribeiro-Paes João Tadeu
Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil.
Univ Estadual PaulistaUNESP, Biological Sciences Department, Av. Dom Antonio, 2100, Campus Universitário, 19806-900 Assis, SP, Brazil.
Exp Toxicol Pathol. 2014 Jul;66(4):195-202. doi: 10.1016/j.etp.2014.01.005. Epub 2014 Feb 18.
The use of anabolic androgenic steroids is often associated with the use of other substances, licit or not, such as nicotine present in the tobacco. The present study investigated for the first time the effects of co-administration of synthetic steroids and nicotine on the ovarian and uterine tissue and fertility of adult female rats. Animals were submitted to treatment groups (n=16/group): nandrolone decanoate (ND; 7.5mg/kg BW/week); testosterone mixture (T; 7.5mg/kg BW/week); nicotine (N; 2.0mg/kg BW/day), and co-administration of ND/N, T/N and ND/T/N. The control group received saline solution daily. The injections were administered subcutaneously for 30 consecutive days. Results demonstrated that all androgenized rats exhibited estral acyclicity and there was suppression of reproductive capacity due to notable ovarian and uterine histological changes. Treatments promoted decrease (p<0.05) in the ovarian weight. Uterine weight increased (p<0.05) in the T and T/N groups, in comparison to control group. ND or T co-administered or not to nicotine promoted intense follicular degeneration, with formation of cysts in the ovaries. High levels of circulating androgens in the ND/T/N group induced the presence of ovarian sex cord-stromal tumors of Sertoli cell pattern. Androgenized females presented endometrial changes characterized by papilliferous or pleated luminal epithelium, oedematous and hemorrhagic stroma and presence of gland cysts. In conclusion, the co-administration of three drugs promoted atypical morphological pattern on the ovaries and uterus of female rats.
合成代谢雄激素类固醇的使用通常与其他物质（无论合法与否）的使用有关，例如烟草中的尼古丁。本研究首次调查了合成类固醇与尼古丁共同给药对成年雌性大鼠卵巢、子宫组织及生育能力的影响。将动物分为治疗组（每组n = 16）：癸酸诺龙（ND；7.5mg/kg体重/周）；睾酮混合物（T；7.5mg/kg体重/周）；尼古丁（N；2.0mg/kg体重/天），以及ND/N、T/N和ND/T/N联合给药组。对照组每天接受生理盐水。连续30天皮下注射给药。结果表明，所有雄激素化大鼠均表现出动情周期紊乱，且由于明显的卵巢和子宫组织学变化，生殖能力受到抑制。治疗导致卵巢重量降低（p<0.05）。与对照组相比，T组和T/N组子宫重量增加（p<0.05）。ND或T与尼古丁联合或不联合给药均导致强烈的卵泡退化，卵巢中形成囊肿。ND/T/N组中高水平的循环雄激素导致出现支持细胞型卵巢性索间质肿瘤。雄激素化雌性大鼠出现子宫内膜变化，其特征为乳头状或褶皱状的管腔上皮、水肿和出血的基质以及腺囊肿的存在。总之，三种药物联合给药导致雌性大鼠卵巢和子宫出现非典型形态模式。
