Cavalcante João Eudes Farias, Martins Solano Dantas, Martins Jonathan Elias Rodrigues, Lobato Jéssica Sales, Sales Yara Silvino, Bittencourt Sara Rany Alexandre, Magalhães Fernanda Vitória Almeida, Menezes Ana Ruth Reinaldo, Oliveira Maria Alice Felipe, Ceccatto Vânia Marilande, Silva Anderson Weiny Barbalho, Salmito-Vanderley Carminda Sandra Brito, Araújo Valdevane Rocha
Programa de Pós-graduação em Ciências Veterinárias - PPGCV, Faculdade de Medicina Veterinária - FAVET, Universidade Estadual do Ceará - UECE, Fortaleza, CE, Brasil.
Laboratório de Biotecnologia da Reprodução de Peixes - LBRP, Núcleo Integrado de Biotecnologia - NIB, Faculdade de Medicina Veterinária - FAVET, Universidade Estadual do Ceará - UECE, Fortaleza, CE, Brasil.
Anim Reprod. 2025 Jan 17;22(1):e20240046. doi: 10.1590/1984-3143-AR2024-0046. eCollection 2025.
This study aimed to compare the effects of nandrolone decanoate on the morphology and physiology of ovarian tissues in two experimental models, Zebrafish and rats, after in vitro cultivation. A total of 136 animals were used ( rats, n=36, and Zebrafish, n=100). In both experiments, the animals were divided into two groups (Control and Deca) and were exposed to nandrolone decanoate for seven weeks. At the end of the administrations, the animals were euthanized, and the tissues were collected for morphological and biochemical analyses. Data were expressed as mean ± SEM. Tukey and Shapiro-Wilk tests were used. ANOVA and chi-square tests were applied for group comparisons. Differences were considered significant when P<0.05. The results showed an increase in body weight in rats, while Zebrafish body weight was decreased. In both experiments, the number of atretic follicles increased throughout the in vitro culture, from day 0 to day 7, in the Control group (CTRLr and CTRLz), while in the DECA group (DECAr and DECAz), atretic follicles were reduced from D0 to D7. The antioxidant environment, represented by increased the thiol content, which was significantly higher on day zero in CTRLz compared to CTRLr. SOD activity increased in Zebrafish (group DECAz), while CAT activity decreased in both models (group DECAr and DECAz). In conclusion, the study demonstrated similarity in ovarian physiology between the models exposed or not exposed to nandrolone decanoate, suggesting that, when convenient, researchers could consider changing the experimental model.
本研究旨在比较癸酸诺龙对斑马鱼和大鼠这两种实验模型的卵巢组织在体外培养后的形态和生理影响。总共使用了136只动物（大鼠，n = 36；斑马鱼，n = 100）。在两个实验中，动物均被分为两组（对照组和癸酸诺龙组），并接受癸酸诺龙处理7周。给药结束时，对动物实施安乐死，并收集组织进行形态学和生化分析。数据以平均值±标准误表示。使用了Tukey检验和Shapiro-Wilk检验。采用方差分析和卡方检验进行组间比较。当P<0.05时，差异被认为具有统计学意义。结果显示，大鼠体重增加，而斑马鱼体重下降。在两个实验中，对照组（CTRLr和CTRLz）从第0天到第7天的体外培养过程中，闭锁卵泡数量增加，而在癸酸诺龙组（DECAr和DECAz）中，从D0到D7闭锁卵泡数量减少。以硫醇含量增加为代表的抗氧化环境，在CTRLz组第0天时显著高于CTRLr组。斑马鱼（DECAz组）超氧化物歧化酶（SOD）活性增加，而在两个模型中过氧化氢酶（CAT）活性均降低（DECAr组和DECAz组）。总之，该研究表明，暴露或未暴露于癸酸诺龙的模型之间在卵巢生理方面具有相似性，这表明在方便时，研究人员可以考虑更换实验模型。
