一种用于检测巨噬细胞表面标志物和非特异性酯酶活性的双重染色方法。
A double staining method for the detection of a macrophage surface marker and nonspecific esterase activity.
作者信息
Leyva-Cobián F, Flores R
机构信息
Servicio de Immunología, Centro Ramón y Cajal, Madrid, Spain.
出版信息
J Immunol Methods. 1988 Jun 28;111(1):147-51. doi: 10.1016/0022-1759(88)90072-5.
A double staining method for the detection on macrophages of a surface marker (Ia molecules) by immunofluorescence and of non-specific esterase activity is described. This method reveals the co-distribution of both markers in macrophages either fixed in suspension or on coverslips with 1% paraformaldehyde. Good resolution of both markers is obtained and the method should be useful for characterizing mononuclear phagocytic cells in heterogeneous cell preparations.
本文描述了一种双重染色方法，用于通过免疫荧光检测巨噬细胞表面标志物（Ia分子）以及非特异性酯酶活性。该方法揭示了在悬浮液中固定或用1%多聚甲醛固定在盖玻片上的巨噬细胞中这两种标志物的共同分布。两种标志物均获得了良好的分辨率，该方法对于鉴定异质性细胞制剂中的单核吞噬细胞应该是有用的。
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