牛骨髓单核吞噬细胞的形态学与细胞化学
Morphology and cytochemistry of bovine bone marrow mononuclear phagocytes.
作者信息
Al-Izzi S A, Maxie M G, Valli V E
出版信息
Can J Comp Med. 1982 Apr;46(2):130-2.
Abstract
Bone marrow samples were collected from five normal calves and mononuclear cells were separated using Ficoll-Hypaque. Mononuclear cells were cultured on coverslips in Leighton tubes for six hours. The adherent cells were differentiated using Wright's and nonspecific esterase stains. Monoblasts, promonocytes and monocytes were present in the proportion of 1:2.31:4.96.
摘要
从五只正常小牛身上采集骨髓样本，并用Ficoll-Hypaque分离单核细胞。将单核细胞接种在莱顿管的盖玻片上培养6小时。用瑞氏染色和非特异性酯酶染色对贴壁细胞进行鉴别。原单核细胞、幼单核细胞和单核细胞的比例为1:2.31:4.96。
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