Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei, Taiwan, Republic of China.
Center for General Education, Toko University, Taiwan, Republic of China.
J Air Waste Manag Assoc. 2013 Dec;63(12):1447-56. doi: 10.1080/10962247.2013.827604.
The main objective of this study is to apply neutral electrolyzed water (NEW) spraying to inactivate bioaerosols. We evaluated the inactivation efficiency of NEW applied to inactivate two airborne bacterial Escherichia coli and Bacillus subtilis aerosols inside an environmental-controlled chamber in the study. Generated with electrolyzing 6.15 M sodium chloride brine, the NEW with free available chlorine (FAC) concentration 50, 100, and 200 ppm was pumped with an air pressure of 70 kg/cm2 through nozzle into the chamber to inactive E. coli and B. subtilis aerosols precontaminated air (initial counts of 3 x 10(4) colony-forming units [CFU]/m3). Bacterial aerosols were collected and cultured from chamber before and after NEW spray. The air exchange rate (ACH, hr(-1)) of the chamber was set to simulate fresh air ventilating dilution of indoor environment. First-order concentration decaying coefficients (Ka, min(-1)) of both bacterial aerosols were measured as an index of NEW inactivation efficiency. The result shows that higher FAC concentration of NEW spray caused better inactivation efficiency. The Ka values under ACH 1.0 hr(-1) were 0.537 and 0.598 for E. coli of FAC 50 and 100 ppm spraying, respectively. The Ka values of FAC 100 ppm and 200 ppm spraying for B. subtilis were 0.063 and 0.085 under ACH 1.0 hr(-1), respectively. The results indicated that NEW spray is likely to be effective in inactivation of bacterial airborne contamination. Moreover, it is observed in the study that the increase of ventilation rate and the use of a larger orifice-size nozzle may facilitate the inactivation efficiency.
本研究的主要目的是应用中性电解水（NEW）喷雾来灭活生物气溶胶。我们评估了在环境控制室内，将 NEW 应用于灭活两种空气传播细菌大肠杆菌和枯草芽孢杆菌气溶胶的灭活效率。NEW 是通过电解 6.15 M 氯化钠盐水产生的，具有 50、100 和 200 ppm 的游离有效氯（FAC）浓度，在 70 kg/cm2 的气压下通过喷嘴泵入室内，以灭活预先污染空气（初始计数为 3 x 10(4) 个菌落形成单位 [CFU]/m3）的大肠杆菌和枯草芽孢杆菌气溶胶。在 NEW 喷雾前后，从室内收集和培养细菌气溶胶。室内的空气交换率（ACH，hr(-1)）设定为模拟室内环境的新风通风稀释。细菌气溶胶的一阶浓度衰减系数（Ka，min(-1)）被用作 NEW 灭活效率的指标。结果表明，NEW 喷雾的更高 FAC 浓度导致更好的灭活效率。ACH 为 1.0 hr(-1)时，FAC 为 50 和 100 ppm 喷雾的大肠杆菌的 Ka 值分别为 0.537 和 0.598。ACH 为 1.0 hr(-1)时，FAC 为 100 和 200 ppm 喷雾的枯草芽孢杆菌的 Ka 值分别为 0.063 和 0.085。结果表明，NEW 喷雾可能有效灭活空气中的细菌污染。此外，在研究中观察到，增加通风率和使用较大的喷嘴孔径可能有助于提高灭活效率。
