Berman P W, Groopman J E, Gregory T, Clapham P R, Weiss R A, Ferriani R, Riddle L, Shimasaki C, Lucas C, Lasky L A
Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080.
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5200-4. doi: 10.1073/pnas.85.14.5200.
The major envelope glycoprotein, gp120, of human immunodeficiency virus type 1 (HIV-1) was purified from a Chinese hamster ovary cell line transfected with a truncated form of the HIV-1 env gene. The recombinant glycoprotein (rgp120) was formulated with aluminum hydroxide adjuvant and was used to immunize chimpanzees. The recombinant preparation was effective in eliciting cellular and humoral immunity as well as immunologic memory. Anti-rgp 120 antibodies reacted with authentic viral gp120 in immunological blot assays and were able to neutralize HIV-1 infectivity in vitro. Sera from the rgp120-immunized animals were able to neutralize HIV-1 pseudotypes of vesicular stomatitis virus prepared from the IIIB isolate, from which the gene encoding rgp120 was derived, as well as two heterologous isolates, ARV-2 and RF. The immune response elicited against the rgp120 was not effective in preventing viral infection after intravenous challenge with HIV-1. The implications of these results on HIV-1 vaccine development are discussed.
从转染了截短形式的HIV-1 env基因的中国仓鼠卵巢细胞系中纯化了1型人类免疫缺陷病毒（HIV-1）的主要包膜糖蛋白gp120。重组糖蛋白（rgp120）与氢氧化铝佐剂混合配制，并用于免疫黑猩猩。该重组制剂在引发细胞免疫和体液免疫以及免疫记忆方面有效。抗rgp120抗体在免疫印迹试验中与天然病毒gp120发生反应，并能够在体外中和HIV-1的感染性。来自用rgp120免疫的动物的血清能够中和由制备rgp120基因的IIIB分离株以及两种异源分离株ARV-2和RF制备的水疱性口炎病毒的HIV-1假型。在用HIV-1静脉内攻击后，针对rgp120引发的免疫反应在预防病毒感染方面无效。讨论了这些结果对HIV-1疫苗开发的意义。
