a School of Chemistry and Chemical Engineering , Southeast University , Nanjing , PR China.
Biofouling. 2014;30(3):313-22. doi: 10.1080/08927014.2013.871540. Epub 2014 Feb 21.
In this work, antibacterial and anti-adhesive polymeric thin films were constructed on polyacrylonitrile (PAN) nanofibrous membranes in order to extend their applications. Polyhexamethylene guanidine hydrochloride (PHGH) as an antibacterial agent and heparin (HP) as an anti-adhesive agent have been successfully coated onto the membranes via a layer-by-layer (LBL) assembly technique confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), energy-dispersive spectroscopy (EDS) and scanning electron microscopy (SEM). The antibacterial properties of LBL-functionalized PAN nanofibrous membranes were evaluated using the Gram-positive bacterium Staphylococcus aureus and the Gram-negative Escherichia coli. Furthermore, the dependence of the antibacterial activity and anti-biofouling performance on the number of layers in the LBL films was investigated quantitatively. It was found that these LBL-modified nanofibrous membranes possessed high antibacterial activities, easy-cleaning properties and stability under physiological conditions, thus qualifying them as candidates for anti-biofouling coatings.
本工作旨在构建聚丙腈（PAN）纳米纤维膜上的抗菌和抗黏附聚合薄膜，以扩展其应用。通过层层自组装（LBL）技术，成功地将盐酸聚六亚甲基胍（PHGH）作为抗菌剂和肝素（HP）作为抗黏附剂涂覆到膜上，这一点通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、能谱（EDS）和扫描电子显微镜（SEM）得到证实。使用革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌评估了 LBL 功能化 PAN 纳米纤维膜的抗菌性能。此外，定量研究了 LBL 薄膜层数对抗菌活性和抗生物污染性能的依赖性。结果表明，这些 LBL 修饰的纳米纤维膜具有高抗菌活性、易清洁性和在生理条件下的稳定性，因此它们有资格作为抗生物污染涂层的候选材料。
