Zhou Bin, Li Yan, Deng Hongbing, Hu Ying, Li Bin
College of Food Science and technology, Huazhong Agriculture University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Colloids Surf B Biointerfaces. 2014 Apr 1;116:432-8. doi: 10.1016/j.colsurfb.2014.01.016. Epub 2014 Jan 23.
Negatively charged gold nanoparticles (GNP) and positively charged lysozyme (Lys) were alternately deposited on negatively charged cellulose mats via layer-by-layer (LBL) self-assembly technique. The fabricated multilayer films were characterized by energy-dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectra (FT-IR), and wide-angle X-ray diffraction (XRD). Morphology of the LBL film coated mats was observed by scanning electron microscopy (SEM). Thermal degradation properties were investigated by differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA). Additionally, the result of microbial inhibition assay indicated that the composite nanofibrous mats had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus, which could be used for antimicrobial packing, tissue engineering, wound dressing, etc.
通过层层（LBL）自组装技术，将带负电荷的金纳米颗粒（GNP）和带正电荷的溶菌酶（Lys）交替沉积在带负电荷的纤维素垫上。通过能量色散X射线（EDX）、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FT-IR）和广角X射线衍射（XRD）对制备的多层膜进行了表征。通过扫描电子显微镜（SEM）观察了LBL膜包覆垫的形态。通过差示扫描量热法（DSC）和热重分析（TGA）研究了热降解性能。此外，微生物抑制试验结果表明，复合纳米纤维垫对大肠杆菌和金黄色葡萄球菌具有优异的抗菌活性，可用于抗菌包装、组织工程、伤口敷料等。
