Parravicini C L, Klatzmann D, Jaffray P, Costanzi G, Gluckman J C
5th Chair of Pathological Anatomy and Histology, University of Milano, Hospital L. Sacco, Italy.
AIDS. 1988 Jun;2(3):171-7.
The immunohistochemical reactivity of four monoclonal antibodies (MAbs): CVK, 49-5, 49-6 and 63-FH2, raised against the p18 protein of HIV-1 was assessed in tissues obtained from HIV-infected and uninfected individuals. As already reported, all the MAbs specifically labelled follicular dendritic cells (FDC) in lymph nodes from HIV-infected patients with lymphadenopathy, and cells of microglial nodules in the brain from patients with AIDS-related encephalopathy. However, cross-reactivity with normal uninfected tissues was also observed: epithelial cells of the skin, the thymus and tonsils with CVK, and astrocytes in the brain of 49-6 and 63-FH2. Such cross-reactivities suggest that 'molecular mimicry' could exist between p18 of HIV and normal constituents of human cells. This phenomenon could be relevant for the diagnostic use of anti-p18 MAbs on pathological specimens, and it could be of importance in the pathophysiology of HIV infection.
评估了四种针对HIV-1 p18蛋白产生的单克隆抗体（MAb）：CVK、49-5、49-6和63-FH2，在从HIV感染和未感染个体获取的组织中的免疫组化反应性。如先前报道，所有这些单克隆抗体均特异性标记了来自患有淋巴结病的HIV感染患者淋巴结中的滤泡树突状细胞（FDC），以及来自患有艾滋病相关脑病患者大脑中的小胶质结节细胞。然而，也观察到了与正常未感染组织的交叉反应：CVK与皮肤、胸腺和扁桃体的上皮细胞发生交叉反应，49-6和63-FH2与大脑中的星形胶质细胞发生交叉反应。这种交叉反应表明HIV的p18与人细胞的正常成分之间可能存在“分子模拟”。这种现象可能与抗p18单克隆抗体在病理标本诊断中的应用有关，并且在HIV感染的病理生理学中可能具有重要意义。
