Austbø Lars, Aas Ida Bergva, König Melanie, Weli Simon Chioma, Syed Mohasina, Falk Knut, Koppang Erling Olaf
Department of Basic Science and Aquatic Medicine, The Norwegian School of Veterinary Science, Ullevålsveien 72, P.O. Box 8146 Dep, 0033 Oslo, Norway.
Norwegian Veterinary Institute, Ullevålsveien 68, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
Dev Comp Immunol. 2014 Jul;45(1):107-14. doi: 10.1016/j.dci.2014.02.007. Epub 2014 Feb 19.
Previously, it has been assumed that fish lack organized mucosa-associated lymphoid structures. Recently, an interbranchial lymphoid tissue (ILT) was described in salmonid gills at a site with substantial exposure to antigen. In this study, immune responses were examined in gills, mid-kidney and the laser-dissected ILT of Atlantic salmon (Salmo salar L.) infected with infectious salmon anaemia virus (ISAV). A strong innate response was observed in gills and mid-kidney and even in the laser-dissected ILT, despite the fact that no virus could be traced in this tissue. A small delayed increase in IgT transcripts, exclusively in the ILT, could indicate that this tissue has a role as a secondary lymphoid organ with clonal expansion of IgT expressing B-cells. Compared to the other examined tissues, gills displayed the earliest replication of the virus, further supporting this tissue as the main entry route for infection with ISAV.
以前，人们一直认为鱼类缺乏有组织的黏膜相关淋巴组织。最近，在鲑科鱼类的鳃中，于一个大量接触抗原的部位发现了鳃间淋巴组织（ILT）。在本研究中，对感染传染性鲑鱼贫血病毒（ISAV）的大西洋鲑（Salmo salar L.）的鳃、中肾以及激光切割的ILT中的免疫反应进行了检测。尽管在该组织中未检测到病毒，但在鳃、中肾甚至激光切割的ILT中均观察到了强烈的先天免疫反应。仅在ILT中出现的IgT转录本的小幅延迟增加，可能表明该组织作为二级淋巴器官，具有表达IgT的B细胞克隆扩增的作用。与其他检测组织相比，鳃中病毒的复制最早出现，这进一步支持了鳃作为ISAV感染主要入口途径的观点。
