Gerttula S, Jin Y S, Anderson K V
Department of Molecular Biology, University of California, Berkeley 94720.
Genetics. 1988 May;119(1):123-33. doi: 10.1093/genetics/119.1.123.
Maternal expression of the Toll gene is required for the production and the correct spatial organization of all lateral and ventral structures of the Drosophila embryo. We show here that the Toll gene is transcribed zygotically in the embryo and that zygotic expression is important for the viability of the larva. Both genetic and molecular data indicate that the zygotic Toll product has the same biochemical activity as the maternal product. The spatial distribution of the Toll transcript in the embryo was analyzed. In contrast to the uniform distribution of the maternal RNA, the zygotic Toll RNA is present in a complex spatial and temporal pattern in the embryo. A striking feature of this pattern is the correlation of the regions of invaginating cells with sites of accumulation of zygotic Toll RNA.
Toll基因的母体表达对于果蝇胚胎所有侧部和腹部结构的产生及正确的空间组织是必需的。我们在此表明，Toll基因在胚胎中进行合子转录，并且合子表达对于幼虫的生存能力很重要。遗传和分子数据均表明，合子Toll产物与母体产物具有相同的生化活性。分析了胚胎中Toll转录本的空间分布。与母体RNA的均匀分布不同，合子Toll RNA以复杂的时空模式存在于胚胎中。这种模式的一个显著特征是内陷细胞区域与合子Toll RNA积累位点之间的相关性。
