Koo Kyo-in, Kim Sang Bok, Kim Keekyoung, Oh Jonghyun
Department of Biomedical Engineering, University of Ulsan, Ulsan, 680-749, Republic of Korea,
Biotechnol Lett. 2014 May;36(5):1089-94. doi: 10.1007/s10529-014-1453-1. Epub 2014 Feb 22.
A mini-microscope-based system for multisite detection of cardiovascular toxicity was developed. The mini-microscope consisted of an image sensor and lens module extracted from an inexpensive webcam. The flipped lens module enabled cells to be magnified and monitored during testing. The portability and compactness of this system enables short-term and potential long-term experimentation inside a conventional incubator. The toxicity test results demonstrated that the normalized beating rates of cardiac muscle cells selected from multiple regions increased over time when treated with 100 nM isoprenaline. The presented system could be a promising cost-effective cell-based testing tool for discovering and screening drugs.
开发了一种基于微型显微镜的心血管毒性多部位检测系统。该微型显微镜由从廉价网络摄像头提取的图像传感器和镜头模块组成。翻转的镜头模块能够在测试过程中对细胞进行放大和监测。该系统的便携性和紧凑性使其能够在传统培养箱内进行短期和潜在的长期实验。毒性测试结果表明，用100 nM异丙肾上腺素处理后，从多个区域选取的心肌细胞的标准化搏动率随时间增加。所提出的系统可能是一种很有前景的、具有成本效益的基于细胞的药物发现和筛选测试工具。
