Tel Aviv University, Department of Biomedical Engineering, Tel-Aviv 69978, Israel.
J Biomed Opt. 2013 Oct;18(10):101310. doi: 10.1117/1.JBO.18.10.101310.
An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extract the statistical data of their movement at once. The presented experimental results also include investigation of the effect of isoproteranol on cell contraction process.
提出了一种用于远程非接触式测量大量心肌细胞机械收缩的无透镜配置。所有的心肌细胞都取自大鼠，测量是在体外模式下进行的。该光学方法基于对无透镜显微镜配置中产生的二次反射散斑图案的时间分析。处理包括分析当细胞培养物被激光束照射时在其顶部产生的二次散斑图案的运动和统计变化。所提出系统的主要优点是能够同时测量许多细胞（约 1000 个细胞）并立即提取它们运动的统计数据。所呈现的实验结果还包括异丙肾上腺素对细胞收缩过程的影响的研究。
