Petrović S, Takić M, Arsić A, Vučić V, Drakulić D, Milošević M, Glibetić M
Institute for Medical Research University of Belgrade, Centre of Research Excellence in Nutrition and Metabolism, Belgrade, Serbia.
Physiol Res. 2014;63(3):331-9. doi: 10.33549/physiolres.932590. Epub 2014 Feb 24.
The effects of 8-days treatment with 17alpha-estradiol (33.3 microg/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of delta9-desaturases and progesterone of delta5-desaturase in BCO group, with no effects in INT rats.
在完整（INT）和双侧颈总动脉闭塞（BCO）的雄性Wistar大鼠中，研究了17α-雌二醇（33.3微克/千克）和孕酮（1.7毫克/千克）8天治疗对血浆脂质和血浆磷脂脂肪酸组成的影响。雌二醇治疗后，BCO大鼠的甘油三酯水平显著降低。两种激素均提高了INT组中18:1n-7脂肪酸的比例，但在BCO组中未能产生这种效果。雌二醇增加了完整组中22:5n-3和总n-3多不饱和脂肪酸（PUFA）的含量，并降低了BCO大鼠中18:2n-6的含量。孕酮治疗组的总n-3水平显著低于雌二醇治疗的BCO大鼠。鉴于n-3 PUFA对细胞和组织功能有许多有益作用，而n-6 PUFA大多具有相反作用，在INT和BCO动物中，雌二醇而非孕酮可改善血浆脂质和磷脂脂肪酸谱。雌二醇显著提高了BCO组中δ9-去饱和酶的估计活性和孕酮的δ5-去饱和酶活性，而对INT大鼠无影响。
