Department of Functional Morphology, Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 2014;63(Suppl 1):S119-31. doi: 10.33549/physiolres.932623.
Thyroid hormones (THs) play multiple roles in the organism and alterations of their levels can result in many pathological changes. Currently, we use hyperthyroid and hypothyroid rats as "models of a diseased organism" and analyze whether n-3 polyunsaturated fatty acids (n-3 PUFA) administration can ameliorate TH-induced pathophysiological changes. We investigate myosin heavy chain composition, calsequestrin levels, changes in cardiac tissue remodeling and cell-to-cell communication, expression of protein kinases, mitochondrial functions, oxidative stress markers and cell death, changes in serum lipid levels, activities of key enzymes of thyroid hormone metabolism, activity of acetylcholine esterase and membrane anisotropy, as well as mobile behavior and thermal sensitivity. Additionally we also mention our pilot experiments dealing with the effect of statin administration on skeletal muscles and sensory functions. As THs and n-3 PUFA possess multiple sites of potential action, we hope that our complex research will contribute to a better understanding of their actions, which can be useful in the treatment of different pathophysiological events including cardiac insufficiency in humans.
甲状腺激素 (THs) 在生物体中发挥多种作用，其水平的改变可导致许多病理变化。目前，我们使用甲状腺功能亢进和甲状腺功能减退的大鼠作为“患病机体的模型”，并分析 n-3 多不饱和脂肪酸 (n-3 PUFA) 的给药是否可以改善 TH 引起的病理生理变化。我们研究肌球蛋白重链组成、钙网蛋白水平、心脏组织重构和细胞间通讯的变化、蛋白激酶的表达、线粒体功能、氧化应激标志物和细胞死亡、血清脂质水平的变化、甲状腺激素代谢关键酶的活性、乙酰胆碱酯酶和膜各向异性的活性，以及移动行为和热敏感性。此外，我们还提到了我们的初步实验，涉及他汀类药物给药对骨骼肌和感觉功能的影响。由于 THs 和 n-3 PUFA 具有多个潜在作用部位，我们希望我们的综合研究将有助于更好地了解它们的作用，这对于治疗包括人类心力衰竭在内的不同病理生理事件可能是有用的。
