Department of Biochemistry, Faculty of Medicine, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), 67630, Esenköy, Kozlu, Zonguldak, Turkey.
BMC Neurosci. 2014 Feb 24;15:33. doi: 10.1186/1471-2202-15-33.
Cardiac complications are often developed after subarachnoid hemorrhage (SAH) and may cause sudden death of the patient. There are reports in the literature addressing ischemia modified albumin (IMA) as an early and useful marker in the diagnosis of ischemic heart events. The aim of this study is to evaluate serum IMA by using the albumin cobalt binding (ACB) test in the first, second, and seventh days of experimental SAH in rats.Twenty-eight Wistar albino rats were divided into four groups each consisting of seven animals. These were classified as control group, 1st, 2nd and 7th day SAH groups. SAH was done by transclival basilar artery puncture. Blood samples were collected under anesthesia from the left ventricles of the heart using the cardiac puncture method for IMA measurement. Histopathological examinations were performed on the heart and lung tissues. Albumin with by colorimetric, creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) were determined on an automatic analyser using the enzymatic method. IMA using by ACB test was detected with spectrophotometer.
Serum IMA (p = 0.044) in seventh day of SAH were higher compared to the control group. Total injury scores of heart and lung tissue, also myocytolysis at day 7 were significantly higher than control group (p = 0.001, p = 0.001, p = 0.001), day 1 (p = 0.001, p = 0.001, p = 0.001) and day 2 (p = 0.001, p = 0.007, p = 0.001). A positive correlation between IMA - myocytolysis (r = 0.48, p = 0.008), and between IMA - heart tissue total injury score (r = 0.41, p = 0.029) was found.
The results revealed that increased serum IMA may be related to myocardial stress after SAH.
蛛网膜下腔出血（SAH）后常发生心脏并发症，可导致患者猝死。文献中有报道称缺血修饰白蛋白（IMA）作为缺血性心脏事件诊断的早期有用标志物。本研究旨在通过白蛋白钴结合（ACB）试验评估实验性 SAH 大鼠第 1、2 和第 7 天的血清 IMA。
28 只 Wistar 白化大鼠分为 4 组，每组 7 只。这些动物被分为对照组、第 1、2 和第 7 天 SAH 组。通过经颅底基底动脉穿刺法制作 SAH 模型。通过心脏穿刺法在麻醉下从左心室采集血液样本，用于 IMA 测量。对心脏和肺组织进行组织病理学检查。使用自动分析仪通过酶法测定白蛋白、肌酸激酶（CK）、天门冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）。使用分光光度计检测 ACB 试验中的 IMA。
与对照组相比，第 7 天 SAH 组的血清 IMA （p = 0.044）更高。第 7 天心脏和肺组织的总损伤评分以及肌溶解程度明显高于对照组（p = 0.001、p = 0.001、p = 0.001）、第 1 天（p = 0.001、p = 0.001、p = 0.001）和第 2 天（p = 0.001、p = 0.007、p = 0.001）。IMA-肌溶解（r = 0.48，p = 0.008）和 IMA-心脏组织总损伤评分（r = 0.41，p = 0.029）之间存在正相关。
结果表明，血清 IMA 的增加可能与 SAH 后心肌应激有关。
