Tettelin Hervé, Davidson Rebecca M, Agrawal Sonia, Aitken Moira L, Shallom Shamira, Hasan Nabeeh A, Strong Michael, de Moura Vinicius Calado Nogueira, De Groote Mary Ann, Duarte Rafael S, Hine Erin, Parankush Sushma, Su Qi, Daugherty Sean C, Fraser Claire M, Brown-Elliott Barbara A, Wallace Richard J, Holland Steven M, Sampaio Elizabeth P, Olivier Kenneth N, Jackson Mary, Zelazny Adrian M
Emerg Infect Dis. 2014 Mar;20(3):364-71. doi: 10.3201/eid2003.131106.
Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol.
对从美国一家囊性纤维化中心的脓肿分枝杆菌马西利亚亚种感染暴发期间患者中分离出的3株近期测序菌株，与来自英国一家囊性纤维化中心暴发的6株菌株以及全球菌株进行了比较。来自这两起囊性纤维化暴发的菌株彼此之间显示出高度相关性，与在巴西一次流行期间引起软组织感染的菌株具有主要相关性。我们在囊性纤维化和软组织暴发菌株中鉴定出独特的单核苷酸多态性，仅在囊性纤维化暴发菌株中存在单独的单核苷酸多态性，并且每个分离株亚组都有独特的基因组特征。我们的研究结果强调了将脓肿分枝杆菌鉴定到亚种水平以及筛查所有囊性纤维化分离株与这些暴发菌株相关性的必要性。我们提出了2种诊断策略，即使用rpoB和secA1基因的部分测序以及多位点序列分型方案。
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