Department of Skin and Allergic Diseases, Skin and Allergy Hospital, Helsinki University Central Hospital, Helsinki, Finland.
Department of Skin and Allergic Diseases, Skin and Allergy Hospital, Helsinki University Central Hospital, Helsinki, Finland.
J Allergy Clin Immunol Pract. 2013 Nov-Dec;1(6):623-31.e1. doi: 10.1016/j.jaip.2013.07.010. Epub 2013 Sep 26.
Hypersensitivity to raw fruits and vegetables is often associated with respiratory allergy to birch (Betula verrucosa) pollen and is considered to be the most prevalent form of food allergy in adults sensitized to birch pollen.
The aim of the study was to investigate the association of clinical allergy and IgE profiles in individuals with birch pollen allergy and hypersensitivity to raw fruits and vegetables.
A total of 59 adults with clinical and skin prick test confirmed birch pollen allergy were included in the study. All the subjects were interviewed by using a structured questionnaire and were examined in vivo by the open test, with the appropriate fruits and vegetables. ImmunoCAP and ImmunoCAP ISAC were used as in vitro diagnostics to assess sensitization profiles for each individual, and principal components analysis was used to analyze the IgE data sets.
Of 59 individuals, 54 (92%) had positive prick-prick test with raw potato, carrot, apple, and/or hazelnut, and the skin prick test was always positive when the corresponding skin challenge was defined as positive. Specific IgE in the ImmunoCAP and inhibition assays with rMal d 1 and rBet v 1 demonstrated that Bet v 1 is driving the sensitization against pathogenesis related-10 proteins. However, positive IgE in vitro results could not be used to predict clinical reactivity to raw fruits and vegetables.
The present study showed that component-based IgE profiling does not enhance the diagnostic potential in case of pollen-food syndrome, which may be associated with other as yet unidentified components.
对生果和蔬菜的过敏反应通常与桦树（Betula verrucosa）花粉的呼吸道过敏有关，被认为是桦树花粉致敏成人中最常见的食物过敏形式。
本研究旨在调查桦树花粉过敏和生食水果和蔬菜过敏个体的临床过敏和 IgE 谱之间的关系。
共纳入 59 名经临床和皮肤点刺试验证实的桦树花粉过敏成人。所有受试者均通过结构化问卷进行访谈，并通过开放测试进行体内检查，使用适当的水果和蔬菜。免疫 CAP 和免疫 CAP ISAC 用于评估每个个体的致敏谱，并使用主成分分析对 IgE 数据集进行分析。
59 名个体中，54 名（92%）对生土豆、胡萝卜、苹果和/或榛子的划痕-划痕试验呈阳性，当相应的皮肤挑战被定义为阳性时，皮肤点刺试验总是阳性。免疫 CAP 中的特异性 IgE 和 rMal d 1 和 rBet v 1 的抑制试验表明，Bet v 1 是导致对病程相关 10 蛋白致敏的原因。然而，体外阳性 IgE 结果不能用于预测生食水果和蔬菜的临床反应性。
本研究表明，基于成分的 IgE 分析并不能增强花粉-食物综合征的诊断潜力，这可能与其他尚未确定的成分有关。
