Department of Bioscience and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, Maharashtra, India,
Arch Microbiol. 2014 Apr;196(4):289-94. doi: 10.1007/s00203-014-0967-1. Epub 2014 Feb 25.
Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process of transcription and translation. The effect of an upshift in the nutritional content of the medium on the cost process of natural lac proteins is not looked upon. Which process of gene expression, transcription, or translation is more important for the cost process is not clear. The current study indicated that cost process is not associated with the upshift mechanism. Cost process was not observed in presence of rifampicin but was detected in existence of chloramphenicol. The current study will help in better understanding of the cost process.
已知表达不必要的蛋白质会降低大肠杆菌的生长速度。由于细胞资源从制造必需蛋白质转移，这种生长速度的降低称为代价。细胞资源取决于细胞的大分子含量。代价现象存在于转录和翻译过程中。上调培养基的营养成分对天然 lac 蛋白的代价过程的影响尚未得到研究。对于代价过程，基因表达的转录或翻译哪个过程更为重要尚不清楚。本研究表明，代价过程与上调机制无关。在存在利福平的情况下未观察到代价过程，但在存在氯霉素的情况下检测到了代价过程。本研究将有助于更好地理解代价过程。
