Ma Liu-Feng, Zhang Jian-Min, Huang Geng-Qing, Li Yang, Li Xue-Bao, Zheng Yong
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China.
Mol Biol Rep. 2014 Jul;41(7):4369-79. doi: 10.1007/s11033-014-3308-1. Epub 2014 Feb 25.
Low temperature, drought and salinity are major abiotic stresses that influence survival, productivity and geographical distribution of many important crops across the globe. The C-repeat/dehydration-responsive element binding transcription factors (CBF/DREB) are important proteins involved in response to abiotic stresses in plants. In this study, twenty-one CBF genes were identified in cotton (Gossypium hirsutum) by bioinformatic approach. The twenty-one CBF genes (named as GhCBF1--GhCBF21) were characterized to encode proteins that share high similarity with those plant cold stress-related CBF proteins, which contain the classic AP2 domain of 58 amino acid residues. Phylogenetic analysis revealed that the isolated cotton CBF genes can be classified into 4 groups: GhCBF I, GhCBF II, GhCBF III and GhCBF IV. RT-PCR analysis indicated that GhCBF genes were up-regulated in cotton plants under cold stress. Furthermore, four GhCBF genes were up-regulated in cotton under salinity and drought treatments. Our data provided valuable information for further exploring the roles of the CBF genes in cotton development and in response to cold stress.
低温、干旱和盐度是影响全球许多重要作物生存、生产力和地理分布的主要非生物胁迫。C-重复/脱水响应元件结合转录因子（CBF/DREB）是参与植物对非生物胁迫响应的重要蛋白质。在本研究中，通过生物信息学方法在棉花（陆地棉）中鉴定出21个CBF基因。这21个CBF基因（命名为GhCBF1 - GhCBF21）的特征是编码与那些植物冷胁迫相关CBF蛋白高度相似的蛋白质，这些蛋白含有58个氨基酸残基的经典AP2结构域。系统发育分析表明，分离出的棉花CBF基因可分为4组：GhCBF I、GhCBF II、GhCBF III和GhCBF IV。RT-PCR分析表明，GhCBF基因在冷胁迫下的棉花植株中上调。此外，在盐度和干旱处理下，棉花中有4个GhCBF基因上调。我们的数据为进一步探索CBF基因在棉花发育和对冷胁迫响应中的作用提供了有价值的信息。
