State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China; Nuclear Institute for Food and Agriculture (NIFA), Tarnab, Peshawar, Pakistan.
Gene. 2013 Dec 1;531(2):220-34. doi: 10.1016/j.gene.2013.09.007. Epub 2013 Sep 11.
NAC (NAM, ATAF, and CUC) is a plant-specific transcription factor family with diverse roles in plant development and stress regulation. In this report, stress-responsive NAC genes (GhNAC8-GhNAC17) isolated from cotton (Gossypium hirsutum L.) were characterised in the context of leaf senescence and stress tolerance. The characterisation of NAC genes during leaf senescence has not yet been reported for cotton. Based on the sequence characterisation, these GhNACs could be classified into three groups belonging to three known NAC sub-families. Their predicted amino acid sequences exhibited similarities to NAC genes from other plant species. Senescent leaves were the sites of maximum expression for all GhNAC genes except GhNAC10 and GhNAC13, which showed maximum expression in fibres, collected from 25 days post anthesis (DPA) plants. The ten GhNAC genes displayed differential expression patterns and levels during natural and induced leaf senescence. Quantitative RT-PCR and promoter analyses suggest that these genes are induced by ABA, ethylene, drought, salinity, cold, heat, and other hormonal treatments. These results support a role for cotton GhNAC genes in transcriptional regulation of leaf senescence, stress tolerance and other developmental stages of cotton.
NAC（NAM、ATAF 和 CUC）是一种植物特异性转录因子家族，在植物发育和应激调节中具有多种作用。在本报告中，从棉花（Gossypium hirsutum L.）中分离出的应激响应 NAC 基因（GhNAC8-GhNAC17）在叶片衰老和应激耐受性方面进行了特征描述。棉花叶片衰老过程中 NAC 基因的特征描述尚未见报道。根据序列特征，这些 GhNAC 可分为三组，属于三个已知的 NAC 亚家族。它们预测的氨基酸序列与其他植物物种的 NAC 基因相似。除了 GhNAC10 和 GhNAC13 之外，所有 GhNAC 基因在衰老叶片中的表达量最大，这两个基因在 25 天授粉后（DPA）的纤维中表达量最大。这 10 个 GhNAC 基因在自然和诱导叶片衰老过程中表现出不同的表达模式和水平。定量 RT-PCR 和启动子分析表明，这些基因受 ABA、乙烯、干旱、盐度、寒冷、高温和其他激素处理的诱导。这些结果支持棉花 GhNAC 基因在叶片衰老、应激耐受性和棉花其他发育阶段的转录调控中的作用。
