箭虫生殖器官的透射电子显微镜分析。

TEM analyses of chaetognath reproductive organs.

作者信息

Shinn George L

机构信息

Biology Department, Truman State University, Kirksville, MO, USA.

出版信息

Methods Mol Biol. 2014;1128:111-23. doi: 10.1007/978-1-62703-974-1_7.

DOI:10.1007/978-1-62703-974-1_7

PMID:24567210

Abstract

Transmission electron microscopy (TEM) enables analysis of subcellular organization. It is especially useful for describing the diverse array of cell types in the gonads and embryos of marine invertebrates. Here, I describe methods for preserving and embedding the reproductive organs of marine arrow worms for TEM, including procedures for staining thick sections for light microscopy and thin sections for TEM.

摘要

透射电子显微镜（TEM）能够分析亚细胞结构。它对于描述海洋无脊椎动物性腺和胚胎中多种多样的细胞类型特别有用。在此，我描述了用于保存和包埋海洋箭虫生殖器官以进行透射电子显微镜观察的方法，包括对厚切片进行光学显微镜染色以及对薄切片进行透射电子显微镜观察的步骤。