赞贾尼蝰蛇成熟精子生殖参数研究。

Study of sperm reproductive parameters in mature zanjani viper.

机构信息

Department of Basic Science, Islamic Azad University, Damghan Branch, Damghan, Iran.

Department of Venomous Animals and Antivenin Production, Vaccine and Serum Research Institute, Karaj, Iran.

出版信息

Cell J. 2014 Summer;16(2):111-6. Epub 2014 May 25.

PMID:24567940

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4072076/

Abstract

OBJECTIVE

Zanjani viper (Vipera albicornuta) is an endemic venomous snake in East Azerbai- jan Province, Iran which is medically important due to its application for antivenin production in the laboratory. We need to produce this snake in captivity. This study was conducted to charac- terize mature male Zanjani viper and to evaluate its sperm reproductive parameters.

MATERIALS AND METHODS

This applied- descriptive study was conducted on twenty Zan- jani viper samples collected from Ag Dag Mountain in East Azarbaijan Province, Iran, between September and October 2010. After the snakes were anesthetized and sacrificed humanly, their morphometric specifications and sperm reproductive parameters, including concentration, motility, vitality, morphology, and survival time, were measured.

RESULTS

MORPHOMETRIC SPECIFICATIONS AND EVALUATION OF SPERMS OF THE SNAKE SHOWED THE FOLLOWING INFORMATION: Zanjani male viper, body length of 73.65 ± 4.35 cm, tail length of 5.465 ± 0.48 cm, and mature snakes with testicular volumes of 0.61 ± 0.81 ml (right) and of 0.46 ± 0.17 ml (left). Our findings revealed average sperm concen- tration of 0.47 ± 0.1 ×10(6)ml(-1), motility of 49 -55 %, vitality of 46.11 ± 9.63 %, normal morphology of 61.71 ± 5.3%, and survival time of 6 ± 2 hours at the laboratory tem- perature. Statistical analyses were performed using Student's t test for comparison of two values, and one-way ANOVA was applied where three values were compared.

CONCLUSION

Results suggest that mature Zanjani male viper with mature sperms in its vas deferens is present in late summer and early autumn seasons in Bostanabad County, Iran.

摘要

目的

扎纳尼蝰蛇（Vipera albicornuta）是伊朗东阿塞拜疆省的一种特有毒蛇，由于其在实验室中用于抗蛇毒血清生产，因此具有重要的医学意义。我们需要在人工饲养的条件下繁殖这种蛇。本研究旨在对成熟雄性扎纳尼蝰蛇进行特征描述，并评估其精子生殖参数。

材料和方法

这项应用描述性研究于 2010 年 9 月至 10 月在伊朗东阿塞拜疆省阿格达格山对 20 条扎纳尼蝰蛇样本进行。在蛇被麻醉并人道处死之后，测量了其形态学特征和精子生殖参数，包括浓度、活力、活力、形态和存活时间。

结果

蛇的形态学特征和精子评估结果如下：雄性扎纳尼蝰蛇，体长 73.65±4.35cm，尾长 5.465±0.48cm，成熟蛇的睾丸体积为 0.61±0.81ml（右侧）和 0.46±0.17ml（左侧）。我们的发现显示平均精子浓度为 0.47±0.1×10（6）ml（-1），活力为 49-55%，活力为 46.11±9.63%，正常形态为 61.71±5.3%，在实验室温度下的存活时间为 6±2 小时。使用学生 t 检验比较两个值进行统计分析，对于三个值的比较，则应用单因素方差分析。