Mozafari Sayedeh Zahra, Shiravi Abdolhossein, Todehdehghan Fatemeh
Department of Biology, Islamic Azad University, Damghan branch, Damghan, Iran;
Department of Venomous Animals, Razi Vaccine and serum Research Institute, Karaj, Iran.
Vet Res Forum. 2012 Spring;3(2):119-23.
Reproductive parameters evaluation is considered as helpful tool for gene bank formation in ecological and economically important animals species. Gloydius halys caucasicus is venomous, viviparous pit viper of northwest of Iran. In this research, the spermatic reproductive parameters of this taxon were studied. Twenty six male snakes were collected from Takht-e-Soleiman region between September and October, 2010. Findings revealed that male snakes with body length of 45.07 ± 2.83 cm and body weight of 51.50 ± 10.42 g, and right and left gonads volume of 0.12 ± 0.03 mL and 0.06 ± 0.01 mL are mature ones and sperms concentration in first, middle and final regions of vas deferens duct were, 22.30 ± 19.34 ×10(6) mL(-1), 30.34 ± 11.55 ×10(6) mL(-1), and 37.65 ± 16.46×10(6) mL(-1), respectively. The sperms motility at three regions of duct were 60.53%, 62.07%, and 60.00% and percentage of immotile sperms in these regions were 39.46%, 37.92%, and 39.84%, respectively. Percentage of morphologically normal sperms was 69.23 ± 10.57% and abnormal sperms was 30.76 ± 10.57%; including 12.69 ± 5.25% spiral tailed, 7.33 ± 4.37% coiled tailed and 4.16 ± 2.51% folded tailed sperms. Percentage of live sperms in the first, middle and final regions of duct were 55.76 ± 10.77%, 58.84 ± 12.77%, and 57.69 ± 9.91%, respectively and percentage of dead sperm in these regions were 44.23 ± 10.77%, 41.15 ± 12.77%, and 42.30 ± 9.91%, respectively. Results suggested, mature sperms with acceptable reproductive quality could be collected from Gloydius halys caucasicus snake of Iran between September and October.
生殖参数评估被认为是生态和经济上重要动物物种基因库形成的有用工具。高加索蝰蛇是伊朗西北部有毒的胎生蝰蛇。在本研究中，对该分类单元的精子生殖参数进行了研究。2010年9月至10月间，从塔克特 - 索莱曼地区收集了26条雄蛇。研究结果显示，体长45.07±2.83厘米、体重51.50±10.42克、左右性腺体积分别为0.12±0.03毫升和0.06±0.01毫升的雄蛇已成熟，输精管起始段、中段和末段的精子浓度分别为22.30±19.34×10⁶毫升⁻¹、30.34±11.55×10⁶毫升⁻¹和37.65±16.46×10⁶毫升⁻¹。输精管三个部位的精子活力分别为60.53%、62.07%和60.00%，这些部位的不活动精子百分比分别为39.46%、37.92%和39.84%。形态正常精子的百分比为69.23±10.57%，异常精子为30.76±10.57%；包括12.69±5.25%的螺旋尾精子、7.33±4.37%的卷尾精子和4.16±2.51%的折尾精子。输精管起始段、中段和末段的活精子百分比分别为55.76±10.77%、58.84±12.77%和57.69±9.91%，这些部位的死精子百分比分别为44.23±10.77%、41.15±12.77%和42.30±9.91%。结果表明，9月至10月间可从伊朗的高加索蝰蛇采集到具有可接受生殖质量的成熟精子。
