Lee Cheng-Hung, Hsieh Ming-Jer, Chang Shang-Hung, Lin Yu-Huang, Liu Shih-Jung, Lin Tzu-Yu, Hung Kuo-Chun, Pang Jong-Hwei S, Juang Jyuhn-Huarng
Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine , Tao-Yuan, Taiwan.
ACS Appl Mater Interfaces. 2014 Mar 26;6(6):3979-86. doi: 10.1021/am405329g. Epub 2014 Mar 6.
This work developed biodegradable nanofibrous drug-eluting membranes that provided sustained release of metformin for repairing wounds associated with diabetes. To prepare the biodegradable membranes, poly-d-l-lactide-glycolide (PLGA) and metformin were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and were spun into nanofibrous membranes by electrospinning. An elution method and an HPLC assay were utilized to characterize the in vivo and in vitro release rates of the pharmaceuticals from the membranes. The biodegradable nanofibrous membranes released high concentrations of metformin for more than three weeks. Moreover, nanofibrous metformin-eluting PLGA membranes were more hydrophilic and had a greater water-containing capacity than virgin PLGA fibers. The membranes also improved wound healing and re-epithelialization in diabetic rats relative to the control. The experimental results in this work suggest that nanofibrous metformin-eluting membranes were functionally active in the treatment of diabetic wounds and very effective as accelerators in the early stage of healing of such wounds.
这项工作开发了可生物降解的纳米纤维药物洗脱膜，该膜可实现二甲双胍的持续释放，用于修复与糖尿病相关的伤口。为制备可生物降解膜，首先将聚-d-丙交酯-乙交酯（PLGA）和二甲双胍溶解于1,1,1,3,3,3-六氟-2-丙醇（HFIP）中，然后通过静电纺丝制成纳米纤维膜。采用洗脱方法和高效液相色谱分析法来表征药物从膜中的体内和体外释放速率。可生物降解的纳米纤维膜释放高浓度二甲双胍超过三周。此外，纳米纤维二甲双胍洗脱PLGA膜比原始PLGA纤维更具亲水性且含水量更高。相对于对照组，该膜还改善了糖尿病大鼠的伤口愈合和再上皮化。这项工作的实验结果表明，纳米纤维二甲双胍洗脱膜在治疗糖尿病伤口方面具有功能活性，并且在这类伤口愈合早期作为促进剂非常有效。
