Feng Li-Li, Liu Bing-Xia, Zhong Jin-Yi, Sun Li-Bin, Yu Hong-Sheng
Department of Oncology, the Affiliated Hospital of Qingdao University, Qingdao, China E-mail :
Asian Pac J Cancer Prev. 2014;15(2):737-41. doi: 10.7314/apjcp.2014.15.2.737.
In recent years a wide variety of flavonoids or polyphenolic substances have been reported to possess substantial anti-carcinogenic and antimutagenic activities. Grape proanthocyanidins (GPC) are considered as good examples for which there is evidence of potential roles as anti-carcinogenic agents.
A xenograft model was established using H22 cells subcutaneously injected into mice and used to assess different concentrations of grape proanthocyanidins (GPC) and Endostar. Treatments were maintained for 10 days, then levels of vascular endothelial growth factor (VEGF) and microvessel density (MVD) were examined by immunohistochemistry, while VEGF mRNA was determined by real-time PCR in tumor tissue.
The expression of MVD and VEGF decreased gradually as the concentration of GPC increased.There was a significant positive correlation between MVD and VEGF.
These results suggest that GPC restrains the growth of tumor, possibly by inhibiting tumour angiogenesis.
近年来，据报道多种黄酮类化合物或多酚类物质具有显著的抗癌和抗诱变活性。葡萄原花青素（GPC）被认为是有证据表明其具有潜在抗癌剂作用的良好范例。
建立将H22细胞皮下注射到小鼠体内的异种移植模型，用于评估不同浓度的葡萄原花青素（GPC）和恩度。治疗持续10天，然后通过免疫组织化学检查血管内皮生长因子（VEGF）水平和微血管密度（MVD），同时通过实时PCR测定肿瘤组织中的VEGF mRNA。
随着GPC浓度增加，MVD和VEGF的表达逐渐降低。MVD和VEGF之间存在显著正相关。
这些结果表明，GPC可能通过抑制肿瘤血管生成来抑制肿瘤生长。
