Zhang X K, Wang Z, Lee A, Huang D P, Chiu J F
Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405.
Cancer Lett. 1988 Aug 15;41(2):147-55. doi: 10.1016/0304-3835(88)90111-5.
The expression of a number of proto-oncogenes (myc, erb B, Ha-ras, bas, rel, mos, sis, myb, ki-ras, fms, src and fos) was studied in developing rat liver. Northern blot hybridization shows that cellular counterpart of erb B, Ha-ras, and fos oncogenes were in an early stage of liver development, and the expressions of these proto-oncogenes gradually decreased as the liver developed, while c-myc transcript was found only in the rat fetal liver. The transcripts of these oncogenes were found in high level in Morris hepatoma 7777. Bas proto-oncogene was found in high expression at early stages of rat liver development but was not in hepatoma 7777. The expression of other proto-oncogenes studied (src, fm, rel, mos, sis, myb and ki-ras) did not change significantly during liver development and was almost the same in hepatoma and normal adult liver. Southern blot analysis demonstrates that gene amplification and apparent gene rearrangement were not responsible for the change in expression of erb B, Ha-ras, myc and fos proto-oncogenes. Our study gives further evidence that erb B, myc, Ha-ras and fos proto-oncogenes are involved in the control of cell growth and in the process of rat hepatocarcinogenesis.
在发育中的大鼠肝脏中研究了多种原癌基因（myc、erb B、Ha-ras、bas、rel、mos、sis、myb、ki-ras、fms、src和fos）的表达。Northern印迹杂交显示，erb B、Ha-ras和fos癌基因的细胞对应物在肝脏发育早期存在，并且随着肝脏的发育这些原癌基因的表达逐渐降低，而c-myc转录本仅在大鼠胎肝中发现。这些癌基因的转录本在莫里斯肝癌7777中高水平存在。发现bas原癌基因在大鼠肝脏发育早期高表达，但在肝癌7777中不表达。所研究的其他原癌基因（src、fm、rel、mos、sis、myb和ki-ras）的表达在肝脏发育过程中没有显著变化，并且在肝癌和正常成年肝脏中几乎相同。Southern印迹分析表明，基因扩增和明显的基因重排与erb B、Ha-ras、myc和fos原癌基因表达的变化无关。我们的研究进一步证明，erb B、myc、Ha-ras和fos原癌基因参与细胞生长的调控以及大鼠肝癌发生过程。
