癌基因在人肝癌细胞系中的表达。
Expression of oncogenes in human hepatoma cell lines.
作者信息
Ting L P, Jeng K S, Chou C K, Su T S, Hu C P, Wong F H, Chang H K, Chang C M
机构信息
Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
出版信息
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1988 Aug;21(3):141-50.
The expression of 20 known cellular proto-oncogenes in human well-differentiated hepatoma cell line Hep3B and poorly-differentiated hepatoma cell line HA22T/VGH was studied by Northern blot hybridization. Among the cellular proto-oncogenes examined, both cell lines express protein kinase genes including fps, mos and raf; PDGF B chain sis gene; GTP/GDP binding protein gene Ha-ras and nuclear protein genes including fos and myc. The expression of yes, abl, ros, src, erb-B, erb-A, fms, Ki-ras, myb, rel and bas genes was not detected in both cell lines.
采用Northern印迹杂交法研究了20种已知细胞原癌基因在人高分化肝癌细胞系Hep3B和低分化肝癌细胞系HA22T/VGH中的表达情况。在所检测的细胞原癌基因中，两种细胞系均表达蛋白激酶基因，包括fps、mos和raf；血小板衍生生长因子B链sis基因；GTP/GDP结合蛋白基因Ha-ras以及核蛋白基因，包括fos和myc。在两种细胞系中均未检测到yes、abl、ros、src、erb-B、erb-A、fms、Ki-ras、myb、rel和bas基因的表达。
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