Bortolotto Josiane W, Cognato Giana P, Christoff Raissa R, Roesler Laura N, Leite Carlos E, Kist Luiza W, Bogo Mauricio R, Vianna Monica R, Bonan Carla D
1 Programa de Pós-Graduação em Biologia Celular e Molecular, Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul , Porto Alegre, Brazil .
Zebrafish. 2014 Apr;11(2):142-53. doi: 10.1089/zeb.2013.0923. Epub 2014 Feb 25.
Chronic exposure to paraquat (Pq), a toxic herbicide, can result in Parkinsonian symptoms. This study evaluated the effect of the systemic administration of Pq on locomotion, learning and memory, social interaction, tyrosine hydroxylase (TH) expression, dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) levels, and dopamine transporter (DAT) gene expression in zebrafish. Adult zebrafish received an i.p. injection of either 10 mg/kg (Pq10) or 20 mg/kg (Pq20) of Pq every 3 days for a total of six injections. Locomotion and distance traveled decreased at 24 h after each injection in both treatment doses. In addition, both Pq10- and Pq20-treated animals exhibited differential effects on the absolute turn angle. Nonmotor behaviors were also evaluated, and no changes were observed in anxiety-related behaviors or social interactions in Pq-treated zebrafish. However, Pq-treated animals demonstrated impaired acquisition and consolidation of spatial memory in the Y-maze task. Interestingly, dopamine levels increased while DOPAC levels decreased in the zebrafish brain after both treatments. However, DAT expression decreased in the Pq10-treated group, and there was no change in the Pq20-treated group. The amount of TH protein showed no significant difference in the treated group. Our study establishes a new model to study Parkinson-associated symptoms in zebrafish that have been chronically treated with Pq.
长期接触有毒除草剂百草枯（Pq）会导致帕金森氏症症状。本研究评估了全身施用Pq对斑马鱼运动、学习和记忆、社交互动、酪氨酸羟化酶（TH）表达、多巴胺和3,4-二羟基苯乙酸（DOPAC）水平以及多巴胺转运体（DAT）基因表达的影响。成年斑马鱼每3天腹腔注射10mg/kg（Pq10）或20mg/kg（Pq20）的Pq，共注射6次。两种治疗剂量下，每次注射后24小时运动和游动距离均下降。此外，Pq10和Pq20处理的动物在绝对转弯角度上表现出不同的影响。还评估了非运动行为，在Pq处理的斑马鱼中，焦虑相关行为或社交互动未观察到变化。然而，Pq处理的动物在Y迷宫任务中表现出空间记忆获取和巩固受损。有趣的是，两种处理后斑马鱼脑中多巴胺水平升高而DOPAC水平降低。然而，Pq10处理组中DAT表达降低，Pq20处理组中无变化。处理组中TH蛋白量无显著差异。我们的研究建立了一个新模型，用于研究长期用Pq处理的斑马鱼中与帕金森相关的症状。
