National Isotope Centre, GNS Science, 30 Gracefield Road, Lower Hutt 5040, New Zealand.
J Agric Food Chem. 2014 Mar 26;62(12):2605-14. doi: 10.1021/jf404766f. Epub 2014 Mar 14.
Carbon isotopes (δ(13)C honey and δ(13)C protein) and apparent C-4 sugar contents of 1023 New Zealand honeys from 15 different floral types were analyzed to investigate which New Zealand honey is prone to failing the AOAC 998.12 C-4 sugar test and evaluate the occurrence of false-positive results. Of the 333 honey samples that exceeded the 7% C-4 sugar threshold, 324 samples of these were New Zealand manuka honey (Leptospermum scoparium, 97.2% of all fails found in the study). Three monofloral honeys (ling, kamahi, and tawari) had nine samples (2.8% of all fails found in the study) with apparent C-4 sugars exceeding 7%. All other floral types analyzed did not display C-4 sugar fails. False-positive results were found to occur for higher activity New Zealand manuka honey with a methylglyoxal content >250 mg/kg or a nonperoxide activity >10+, and for some ling, kamahi and tawari honeys. Recommendations for future interpretation of the AOAC 998.12 C-4 sugar method are proposed.
对来自 15 种不同花卉类型的 1023 种新西兰蜂蜜的碳同位素（δ(13)C 蜂蜜和 δ(13)C 蛋白质）和表观 C-4 糖含量进行了分析，以调查哪种新西兰蜂蜜容易导致 AOAC 998.12 C-4 糖测试失败，并评估假阳性结果的发生。在超过 7% C-4 糖阈值的 333 个蜂蜜样品中，这些样品中有 324 个是新西兰麦卢卡蜂蜜（Leptospermum scoparium，占研究中发现的所有失败品的 97.2%）。三种单花蜂蜜（ling、kamahi 和 tawari）有九个样品（占研究中发现的所有失败品的 2.8%）的表观 C-4 糖含量超过 7%。分析的其他所有花卉类型均未显示 C-4 糖失败。发现对于甲基乙二醛含量>250mg/kg 或非过氧化物活性>10+的高活性新西兰麦卢卡蜂蜜，以及某些 ling、kamahi 和 tawari 蜂蜜，会出现假阳性结果。建议对未来 AOAC 998.12 C-4 糖方法的解释进行改进。
