选择钠通道缺失或改变的变异神经母细胞瘤克隆。
Selection of variant neuroblastoma clones with missing or altered sodium channels.
作者信息
West G J, Catterall W A
出版信息
Proc Natl Acad Sci U S A. 1979 Aug;76(8):4136-40. doi: 10.1073/pnas.76.8.4136.
Abstract
Neurotoxins that cause persistent activation of voltage-sensitive sodium channels are highly cytotoxic to electrically excitable neuroblastoma cells. These toxins were used as selective agents to isolate variant neuroblastoma clones with missing or altered sodium channels. Of ten resistant clones analyzed, seven lacked functional sodium channels and one had a specific 40-fold increase Kd for scorpion toxin and altered voltage dependence of scorpion toxin binding. The phenotypes of these cell clones were stable for more than 100 generations, indicating that they were the result of stable genetic change.
摘要
导致电压敏感性钠通道持续激活的神经毒素对电兴奋性神经母细胞瘤细胞具有高度细胞毒性。这些毒素被用作选择剂，以分离钠通道缺失或改变的变异神经母细胞瘤克隆。在分析的10个抗性克隆中，7个缺乏功能性钠通道，1个对蝎毒素的解离常数（Kd）有特定的40倍增加，并且蝎毒素结合的电压依赖性发生改变。这些细胞克隆的表型在100多代中保持稳定，表明它们是稳定遗传变化的结果。
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